摘要
采用谷胱甘肽亲和层析和离子交换层析，从人体胎盘提纯ＧＳＴ－π血制备兔抗ＧＳＴ－π血清，纯化其ＩｇＧ，并建立ＧＳＴ－π双抗夹心ＥＬＩＳＡ法。检测了７３例正常人和１９３例消化道良、恶性肿瘤患者血清ＧＳＴ－π含量。结果示消化道恶性肿瘤患者血清ＧＳＴ－π含量较正常人，良性肿瘤和非肿瘤患者明显增高（Ｐ＜０．０１～０．０００１）。ＧＳＴ－π对原发性肝癌、转移性肝癌、胃癌、结直肠癌和食管癌的诊断阳性率分别为９０．６％、９０．０％、６８．４％、６６．６％和５７．０％，对消化道恶性肿瘤总的诊断敏感性为８０．０％，特异性为９６．０％。
Glutathione S-transferase(GST-
π)Was extracted and purified from human placenta,andthe determlnation of serum GST-π by
double sandwich ELISA was established.The serumGST-π concentration of healthy controls
was 2.06 ± 1.41ng/ml(M±SD).Marked increase inserum GST-π was observed in patients with
hepatocellular carcinoma(51.05 ± 46.51ng/m1),metastatic liver cancer(66.77 ±
56.85ng/m1),gastric cancer(16.92 ±18.03ng/ml),colorectal cancer(15.18 ±14.07ng/ml) and
esophageal cancer(7,776±6.12ng/ml).These results indicate that ELISA method of determining
serum GST-π is useful in serologicaldiagnosis of gastrointestihal malignancies.
出处
《中华消化杂志》
CAS
CSCD
北大核心
1994年第2期92-96,共5页
Chinese Journal of Digestion
关键词
GST
ELISA
胃肿瘤
肝肿瘤
诊断
Human placental glutathione S- transferase
Enzyme-linked immunosorbent
assay
Hepatocellular carcinoma
Gastric cancer
Colorectal cancer.
