摘要
目的研究海洋生物海燕(Asterina pectinifera)的化学成分。方法采用大孔树脂柱色谱、硅胶柱色谱、凝胶柱色谱、制备薄层色谱、高效液相色谱等手段分离纯化,根据理化性质、波谱分析和文献对照的方法对所分离的化合物进行结构鉴定。结果从海燕体内分离并鉴定了9个化合物,分别为:3-十八烷氧基-1,2-丙二醇[3-(octadecyloxy)-1,2-propanediol,1]、正二十碳酸(arachidic acid,2)、胆甾-7-烯-3β-醇(cholesta-7-ene-3β-ol,3)、尿嘧啶(uracil,4)、5α-胆甾烷-3β,4β,6α,7α,8,15β,16β,26-八醇(5α-cholestane-3β,4β,6α,7α,8,15β,16β,26-octol,5)、5α-胆甾烷-3β,6α,8,15α,24-五醇(5α-cholestane-3β,6α,8,15β,24-pentaol,6)、色氨酸(tryptophan,7)、3,4-二羟基苯甲酸(3,4-dihydroxybenzoic acid,8)、pectinioside B([(5α,20R,22R,23S,24S)-22,23-epoxy-3β,6α,20-trihydroxyergost-(9)11-ene 6-O-{β-D-galactopyranosyl-(1→4)-[β-D-quinovopyranosyl-(1→2)]-β-D-glucopyranosyl-(1→4)-[β-D-quinovopyranosyl-(1→2)]-β-D-xylopyranosyl-(1→3)-β-D-quinovopyranoside}-3-O-sodium sulfate],9)。结论化合物2、8为首次从海燕中分离得到,化合物9为首次从中国海域的海星纲中分离得到。首次采用二维核磁技术确定化合物9的结构,并首次对其1H-NMR数据及二维谱数据进行归属。
Objective To study the chemical constituents of the starfish Asterina pectinifera.Methods These compounds were isolated by column chromatography on silica gel,sephadex LH-20 and HPLC.Their structures were identified by spectroscopic analysis and physical-chemical properties.Results Nine compounds were obtained and identified as 3-（octadecyloxy）-1,2-propanediol（1）,arachidic acid（2）,cholesta-7-ene-3β-ol（3）,uracil（4）,5α-cholestane-3β,4β,6α,7α,8,15β,16β,26-octol（5）,5α-cholestane-3β,6α,8,15α,24-pentaol（6）,tryptophan（7）,3,4-dihydroxybenzoic acid（8）,（5α,20R,22R,23S,24S）-22,23-epoxy-3β,6α,20-trihydroxyergost-（9）11-ene 6-O-{β-D-galactopyranosyl-（1→4）-[β-D-fucopyranosyl-（1→2）]-β-D-glucopyranosyl-（1→4）-[β-D-quinovopyranosyl-（1→2）]-β-D-xylopyranosy-l-（1→3）-β-D-quinovopyranoside}-3-O-sodium sulfate（9）.Conclusions Compounds 2 and 8 are obtained from Asterina pectinifera for the first time.1H-NMR spectroscopic data of compound 9 is reported for the first time and this compound is also identified by 2-D NMR for the first time.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2012年第10期765-769,共5页
Journal of Shenyang Pharmaceutical University
基金
教育部新世纪优秀人才支持计划资助项目(NCET-10-0854)
海洋工艺性行业科研专项资助项目(201205022-7)
关键词
海燕
海星
化学成分
结构鉴定
Asterina pectinifera
starfish
chemical constituent
structure identification
