摘要
目的:了解脂质过氧化对实验性肝纤维化的影响。方法:将36只雄性Wistar大鼠分为正常组(N组)、3、6、9周组(A、B、C组),用CCL_4及5％酒精诱导肝纤维化模型,分别在3、6、9周宰杀动物,观察三组肝组织病理改变及检测肝组织中丙二醛(MDA)和羟脯氨酸(HYP)含量变化。结果:A组肝组织中MDA和HYP含量明显高于正常大鼠组(P<0.01)。随染毒时间延长,肝组织中MDA和HYP含量不断增加,三组间有显著性差异(P<0.01)。光镜下,A、B、C组肝组织呈现不同程度的肝纤维化,染毒时间越长纤维化程度越重。肝组织中HYP含量变化与MDA含量变化呈正相关,γ为0.80(P<0.01)。结论:CCL_4及低浓度酒精诱导的肝纤维化与肝脂质过氧化有关。
Objective: To investigate the effects of lipoperoxidation on experimental liver fibrosis. Method: 36 Wistar rats were randomly divided into four groups, i.e. 3 weeks group, 6 weeks group, 9 weeks group and normal group. CCL4 and low dose alcohol were used to induce the model of liver fibrosis. Except the normal group, the other groups were injected with solution of CCL4 in subcutaneous and drank 50% alcohol ad libitum. After 3, 6 and 9 weeks, the animals were killed, respectively, to obtain the specimens of their tissues. The histological changes of livers in every group were observed under light microscope. The level of malondialdehyde (MDA) and hydroxyproline (HYP) were measured by biochemical methods. Result: The levels of MDA and HYP of tested group were higher than those of normal group (P<0. 01). The longer the experimental time treating rats with CCL4 lasted, the higher the levels of these two indexes were and the degree of liver fibrosis was. A relationship between HYP values and MDA values were observed and showed a positive correlation (r = 0. 80,(P<0. 01). Conclusion ; The liver fibrosis induced by CCL4 and alcoho is related with lipoperoxidation of liver.
出处
《西南国防医药》
CAS
2000年第3期139-142,共4页
Medical Journal of National Defending Forces in Southwest China
关键词
脂质过氧化
丙二醛
肝纤维化
羟脯氨酸
lipoperoxidation liver fibrosis malondialdehyde hydroxyproline
