摘要
目的研究4-羟基苯并恶唑-2-酮(4-hydroxy-2-benzoxazolone,HBOA)对四氯化碳所致小鼠急性肝损伤的保护作用,并探讨其疗效机制。方法采用腹腔注射四氯化碳(carbon tetrachloride,CCl4)制备小鼠急性肝损伤模型,HBOA灌胃给药,检测小鼠血清中的乳酸脱氢酶(LDH)活性以及肝组织中过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)含量,并用免疫组化法观察肿瘤坏死因子(TNF-α)的表达情况。结果 HBOA能明显降低CCl4致急性肝损伤小鼠血清LDH活性,同时升高肝组织中CAT、GSH-Px的活性并降低肝组织中TNF-α的表达。结论 HBOA对CCl4所致小鼠急性肝损伤有一定的保护作用。
Objective To study the protective effect of 4-Hydroxy-2-benzoxazolone （HBOA） against liver injury induced by carbon tetrachloride （CCI4） in mice. Methods The acute liver injury model was prepared by the intraperitoneal injection of carbon tetrachloride. The levels of LDH in serum and levels of CAT,GSH-Px in liver tissue were measured and the expression of TNF-a were observed in order to evaluate the protective effects of HBOA. Results Com- pared with control group,the levels of serum LDH and the expression of TNFa in liver tissue were decreased significantly and the levels of CAT,GSH-Px in liver tissue were increased obvi ously. Conclusion HBOA has protective effects against acute hepatic injury induced by CC14 in mice.
出处
《蛇志》
2012年第1期5-7,10,共4页
Journal of Snake
基金
广西自然基金资助项目(编号2009AM4019)
