摘要
目的:探讨重组人脑利钠肽(rhBNP)后适应对兔急性缺血/再灌注(I/R)心肌的保护作用及其机制。方法:将36只健康日本大耳白兔随机分为3组(每组12只)。即假手术组、I/R组(缺血40 min后再灌注180 min)及BNP+I/R组(I/R前5 min以0.01μg/kg静脉给予BNP)。假手术组开胸于左室支下穿线但不结扎,观察180 min结束;I/R组及BNP+I/R组:缺血40 min,分别再灌注180 min后处死。进行心肌酶学CK-MB含量检测,观察心肌HE染色后病理形态变化及BCI-2、Bax表达的检测。结果:与假手术组相比,I/R组和BNP+I/R组CK-MB的水平均显著增高(P<0.01)。与I/R组相比,BNP+I/R组明显减少这些心肌酶的升高(P<0.01)。与假手术组相比,I/R组及BNP+I/R组均可见到凋亡细胞,但BNP+I/R组凋亡细胞明显减少。与假手术组相比,I/R组Bax的表达增加,Bcl-2的表达降低。与I/R组相比,BNP+I/R组Bax的表达明显降低,而Bcl-2的表达明显升高。结论:重组人脑钠尿肽可减少梗死后心肌的损伤;可以增加梗死后心肌细胞抑制凋亡基因(Bcl-2)的表达,从而减少心肌细胞的凋亡。
AIM ： To study the protective effect of rhBNP postconditioning on acute myocardial ischemia/ reperfusion injury in rabbits and its mechanism. METHODS： Thirty-six healthy rabbits were randomly divided into three groups with 12 rabbits in each group： sham-operated group, ischemia-reperfusion group and BNP postconditioning group. Blood vessels were not occluded in sham-operated group, a 40-min occlusion of the left coronary vessel was followed by a 180-min reperfusion of physiological saline in ischemia-reperfusion group and BNP was injected 5 min before and throughout the whole reperfusion in the BNP postconditioning group. Serum CK-MB was detected and recorded. Expressions of Bcl-2 and Bas were examined. RESULTS： Levels of CK-MB markedly increased in ischemia reperfusion group （P 〈 0. 01 ） compared with those in sham-operated group. The elevation of CK-MB obviously reduced in BNP postconditioning group. Myocardial cell apoptosis significantly reduced in BNP postconditioning group compared with that in I/R group. The expression of Bax increased and the expression of Bcl-2 decreased, which were contrary to those in BNP postconditioning group. CONCLUSION： Biochemical examinations show that BNP reduces myocardial infarction injury and increases the expression of Bcl-2, thus lowering myocardial apoptosis.
出处
《心脏杂志》
CAS
2012年第5期569-572,共4页
Chinese Heart Journal
基金
黑龙江省科技厅青年基金项目资助(QC2011C123)
关键词
心肌梗死
缺血/再灌注
脑钠肽后适应
兔
myocardial ischemia reperfusion injury
BNP
apoptosis Bcl-2
Bas
