摘要
目的:探讨清心开窍方有效成分对AD大鼠学习记忆能力及脑组织细胞凋亡基因Bax、Bcl-2 mRNA及胶质纤维酸性蛋白(GFAP)mRNA表达的影响。方法:动物实验采用对照观察法。雄性SD大鼠56只,随机分为正常组、模型组、安理申组、清心开窍方组、皂苷组、多糖组和挥发油组,每组8只。采用双侧杏仁核注射Aβ25-35制造AD大鼠模型,造模后第2天起,分别灌胃给药安理申、清心开窍方水煎剂、皂苷、多糖、挥发油及双蒸水,2周后,观察大鼠水迷宫空间记忆能力,并通过RT-PCR研究脑组织Bax、Bcl-2 mRNA及GFAP mRNA的表达。结果:中药各组能明显改善AD大鼠学习记忆能力,大鼠大脑皮层、海马区Bax、GFAP mRNA表达水平降低,清心开窍方组、皂苷组皮质Bcl-2 mRNA表达水平升高,与模型组比较差异显著(P<0.05,P<0.01)。结论:清心开窍方能显著改善AD大鼠学习记忆的能力,可能是通过降低皮质、海马Bax mRNA及GFAP mRNA表达,升高Bcl-2 mRNA表达发挥作用。
Objective： To study the effect of Qingxin Kalqiao Formula and effective constituents on the expression of Bax, Bcl-2 mRNA and GFAP mRNA in brain of AD rats. Methods ： Animal test used comparison method. Fifty-six male SD rats were randomly divided into seven groups： the normal group, the model group, the Aricept group, the Qingxin Kaiqiao Formula group, the saponin group, the polysaccharide group and the essential oils group, with 8 rats in each group. AD was induced by Aβ25-35 injected into the bilateral amygdale. The next day after molding, every group started to give medicines the Aricept, the Qingxin Kaiqiao Formula Decoction, saponin, polysaccharide, the essential oils and double distilled water, respectively. After 2 weeks, observed the spatial learning and memony ability, the expression of Bax, Bcl-2 mRNA and GFAP mRNA by RT-PCR （reverse transcription-PCR） in the brain of the animal. Results： Learning and memory ability of Qingxin Kaiqiao Formula groups was improved, the expression of Bax, Bcl-2 mRNA and GFAP mRNA decreased in the cortex and hippocampus, the expression of Bcl-2 mRNA of saponin group increased in the cortex, compared with model group, the difference was significant （P〈0.05, P〈0.01）. Conclusion： Qingxin Kaiqiao formula may significantly improved in learning and memory ability of rats with AD by decreasing the expression of Bax, Bcl-2 mRNA and GFAP mRNA in the cortex and hippocampus.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2012年第10期2664-2668,共5页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.30973780)
浙江省自然科学基金项目(No.Y2080273)
温州市科技局资助项目(No.Y20080106)~~
关键词
阿尔茨海默病
清心开窍方
细胞凋亡
胶质纤维酸性蛋白
β-淀粉样蛋白
Alzheimer＇s disease
Qingxin Kaiqiao Formula
Neuron apoptosis
Aglial fibrillary acidic protein
β-amyloid precursor protein
