摘要
以盐酸萘乙二胺为显色剂,利用UV-9200型紫外可见分光光度计,测定了丽江市销售的几种蔬菜中亚硝酸盐的含量,并确定该测定方法的最佳试验条件。结果表明:pH值在3～4,显色温度15～25℃,显色剂用量为1.0 mL,显色时间在15 min后加水定容到50 mL在最大吸收波长538 nm处测定吸光度。进一步对蔬菜储藏过程和不同储藏条件下亚硝酸盐含量的变化趋势情况进行了研究。
The content of nitrite in the different vegetables in storage in Lijiang City was determined by UV-9200 visible spectrophotometry method,and hydrochloric acid naphthylamine was used as the chromogenic reagent.The top conditions,such as acidity,temperature,consumption of chromogenic reagent were investigated.The results showed that the stability was good and the absorbance fluctuates was little at pH value 3～4,the temperature was at 15～25 ℃,the chromogenic reagent was 1.0 mL,then cooled 15 minutes and added water to 50 mL,finally determined at 538 nm.Further researches of the changeable nitrite contents in the different vegetables in storage were made.
出处
《现代农业科技》
2012年第18期291-292,294,共3页
Modern Agricultural Science and Technology
关键词
蔬菜
储藏
亚硝酸盐
分光光度法
vegetables
storage
nitrite
spectrophotometry
