摘要
目的 :研究 p16和Rb基因在mRNA水平的表达及其与肺癌的关系。方法 :制备 p16和Rb基因cDNA探针 ,采用双重原位杂交的方法 ,对 89例肺癌和正常肺组织进行了 p16和Rb基因mRNA表达的定位研究。 结果 :小细胞肺癌 p16mRNA阳性表达率高达 82 4% (14/ 17) ,而非小细胞肺癌阳性率为 45 8% (33/ 72 ) ,两者差异有显著性 (P <0 0 5 )。非小细胞肺癌RbmRNA阳性表达率为 81 9% (5 9/ 72 ) ,而小细胞肺癌阳性率仅 5 9% (1/ 17) ,两者差异有高度显著性 (P <0 0 1)。两种基因在蛋白水平和mRNA水平的表达基本相符。结论 :p16基因mRNA表达的丢失主要发生于非小细胞肺癌 ,Rb基因表达的丢失主要发生于小细胞肺癌。在翻译水平上也存在着引起 p16和Rb基因蛋白失活的可能机制。
Purpose To study p16 and Rb gene mRNA expressions and their relationships to lung cancer. Methods Preparation of p16 and Rb cDNA probes and in situ hybridization were applicated to locate p16 and Rb mRNA expressions in 89 cases of lung cancers and lung tissues. Results The positive expression rate of p16 mRNA in small cell lung cancers was 82 4%(14/17), but 45 8%(33/72) in non small cell lung cancers. The positive expression rate of Rb mRNA in non small cell lung cancers was 81 9%(59/72), but only 5 9%(1/17) in small cell lung cancers. The expressions of these two genes at protein and mRNA levels fundamentally corresponded with each other. Conclusion The loss of p16 mRNA expression mainly takes place in non small cell lung cancers, and loss of Rb mRNA expressions mainly takes place in small cell lung cancers. Maybe there is a mechanism at gene translation level which leads to the inactivation of p16 or Rb gene protein.
出处
《临床与实验病理学杂志》
CAS
CSCD
2000年第3期180-183,共4页
Chinese Journal of Clinical and Experimental Pathology
基金
国家"九五"重点科技攻关项目!(No 9690 60 1 1 8)
国家自然科学基金!(No 39670 71 4 )资助项目
