摘要
目的:研究纤维素酶辅助提取补骨脂中总黄酮的最佳工艺条件。方法:在单因素试验的基础上,采用响应面分析法研究了酶解pH值、酶解温度、酶解时间和酶用量对补骨脂中总黄酮得率的影响。结果:酶法辅助提取补骨脂中总黄酮的最佳条件为pH值4.9,酶解温度46℃,酶解时间150 min,酶用量7.2 mg/g;在该条件下,总黄酮得率验证值与预测值的相对误差为1.16%。结论:优化所得工艺条件简单可行,可用于补骨脂中总黄酮的提取。
Objective：To study the optimum extraction process of total flavonoids from Psoralea corylifolia by cellulose-assisted technique.Methods：Based on single-factor experiments,the effects of pH value,temperature of enzymatic hydrolysis,time of enzymatic hydrolysis and enzyme amount on the extraction yields of total flavonoids from Psoralea corylifolia were studied by response surface methodology.Results：The optimum enzyme-assisted extraction process was：pH value 4.9,temperature 46 ℃,time 150 min and enzyme amount 7.2 mg/g,under this condition,the relative error of the observed and predicted values was 1.16%.Conclusion：The optimum enzyme-assisted extraction process is simple and feasible,the extraction rate of total flavonoids increases by 28% compared with ultrasonic extraction,so it can be used to extract total flavonoids from Psoralea corylifolia.
出处
《中药材》
CAS
CSCD
北大核心
2012年第7期1144-1147,共4页
Journal of Chinese Medicinal Materials
关键词
补骨脂
总黄酮
提取
纤维素酶
响应面法
Psoralea corylifolia L.
Total flavonoids
Extraction
Cellulase
Response surface methodology
