摘要
肿瘤干细胞的分离和鉴定是肿瘤干细胞研究的关键。目前已利用无血清悬浮培养法从视网膜母细胞瘤、脉络膜黑色素瘤、乳腺癌、肺癌、结肠癌等多种肿瘤中成功分离、富集出肿瘤干细胞，使这些肿瘤干细胞的生物学功能和特性逐渐被认识。本文从无血清培养基的构成及其在眼部和部分全身肿瘤干细胞的分离培养中的应用方面进行综述，以期为眼部肿瘤干细胞的进一步研究提供依据。
The isolation and identification of cancer stem cells are the key points in exploring characteristics of cancer stem cells at present. Several species of cancer stem cells, for instance, retinoblastoma tumor stem cells, cancer stem cells of melanoma of choroid, breast cancer stem cells, lung cancer stem cells, colon cancer stem cells, etc. , have been isolated and cultured successfully by serum free medium while their biological functions and characteristics are acquired . This review focus on the application of serum free medium in the research of isolating cancer stem cells, both ocular and general, in terms of providing foundation for further research on ocular cancer stem cells.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
2012年第9期861-864,共4页
Chinese Journal of Ophthalmology
关键词
细胞
培养的
培养基
无血清
肿瘤干细胞
Cells,cultured
Culture media, serum-free
Neoplastic stem ceils
