摘要
本实验采集脐血并分离脐血单核细胞与血清,分别用胎牛血清与自体脐血血清培养人脐血间充质干细胞。通过检测所培养出的成纤维细胞样细胞的表面抗原标记与细胞的成骨、成脂和成心肌细胞分化能力,鉴定所培养的细胞是否为间充质干细胞。成功采集的21例脐血中,有11例用胎牛血清和15例用自体血清培养出了间充质干细胞,表达细胞表面抗原标记CD29、CD44、CD105,不表达CD34、CD45,并成功地诱导成成骨细胞,脂肪细胞和心肌样细胞,说明使用自体血清培养脐血间充质干细胞较使用胎牛血清有较高的成功率,而且,自体血清培养的脐血间充质干细胞也能成功的进行多向诱导分化。
In this study, we collected human umbilical cord blood and separated the autologous serum and mononuclear ceils. Then the human mesenchymal stem cells were cultured with the use of autologous serum and fetal bovine serum, respectively. By identiflng surface markers with flow cytometry and testing the osteogenic, adipogenic and cardiomyocytes differentiation capacity of the cells, we affirmed that the cultured cells are mesenchymal stem cells. Among 21 cases of umbilical cord blood, 11 cases using fetal bovine serum and 15 cases using autologous serum were found to have successfully cultured mesenchymal stem cells from umbilical cord blcod. The UCB-MSCs did not express CD34 and CD45, but they expressed CD29, CD44 and CD105, and they were successfully induced into osteogenic cells, adipogenic cells and cardiomyocytic-like cells. All of these suggest that using autologous serum to culture UCB-MSCs is better than using fetal bovine serum, and the autologous serum cultured UCB-MSCs can also be induced into osteogenic cells, adipogenic cells and cardiomycyote-like cells.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2008年第5期1155-1160,共6页
Journal of Biomedical Engineering
基金
四川省科技厅攻关项目资助(03SG022-002-2)
关键词
脐血间充质干细胞
自体血清诱导分化
Mesenchymal stem ceils from umbilical cord blood （UCB-MSCs） Autologous serum Induceddifferentiation
