摘要
目的制备低分子肝素聚乳酸-羟基乙酸(LWMH-PLGA)缓释微球,观察微球表面形态,检测微球物理性能和体外释药行为。方法采用W1/O/W2复乳溶剂挥发法制作微球;通过扫描电镜观察微球的表面形态结构;利用天青A比色法测试微球中药物的载药量和包封率,并对微球中药物的体外释放行为进行研究。结果微球表面显现较多的孔隙,平均粒径为(2.55±0.94)μm,载药量为(14.79±1.03)%,包封率为(55.7±2.21)%;48 h的体外释放试验表明,LWMH累积释放率达到40%。结论 LWMH-PLGA微球能够稳定地释放药物LWMH,验证了PLGA微球作为LWMH控制释放载体的可行性。
Purpose To prepare LWMH-PLGA microspheres and to investigate the characteristics. Methods The LWMH-PLGA microspheres were prepared by W1/O/W2 multiple emulsion volatilizing method,the morphology was investigated using scanning electron microscope （SEM） and the UV-Vis method was used to establish the regression equation and to detect the drug loading amount and encapsu- lation efficiency,as well as sustained-release profile in vitro. Results The microsphere surfaces seemed to be with sporadic pores and uniform with mean particle size of （2.55 ± 0.94） μm. The drug loading a- mount and encapsulation efficiency were （ 14.79 ±1.03 ） % and （55.7 ± 2.21 ） %, respectively. The ac- cumulative release ratio was up to 40% in the continuous PLGA microspheres have better pharmaceutical properties period of two days. Conclusion The LWMH- and sustained release effect in vitro
出处
《中国生化药物杂志》
CAS
CSCD
北大核心
2012年第5期600-602,共3页
Chinese Journal of Biochemical Pharmaceutics
