摘要
[目的]探讨球孢白僵菌菌株的生物学特性与其对家蚕致病力的关系,为家蚕白僵病的防治提供科学依据。[方法]从我国各地收集6个白僵菌杀虫剂,并从广西养蚕区采集3个僵蚕样品,对从中分离纯化的菌株进行形态观察和分子鉴定,同时对家蚕进行致病力测定,比较各菌株菌落直径、产孢量、胞外蛋白酶产生水平等生物学特征指标的差异。[结果]获得的9个分离菌株均为球孢白僵菌(Beauveria bassiana Vuillemin);各菌株对家蚕都有较强致病力,且致病力有一定差异;各菌株菌落生长直径、产孢量及胞外蛋白酶活性均存在较大差异,并与其对家蚕的致病力之间存在相关关系。[结论]各菌株产孢量和胞外蛋白酶活性与其对家蚕的致病力之间存在显著正相关。
[ Objective] This study was to investigate the relationship between biological characteristics of Beauveria bassiana （ Bals. ） Vuill and pathogenicity of Bombyx mori L. , with the aim to provide scientific basis for the control of white museardine in Bombyx mori L.. [ Meth- od ] The strains isolated and purified from the 6 Beauveria bassiana biocontrol agents fi＇om all over the country and the 3 white muscardine silk- worms collected from Guangxi provincial silkworm rearing areas were identified by the morphological observation and molecular biology technol- ogy, The pathogenicity of silkworms to B. t^assaina was determined, and the blological characteristics such as growth diameter, spore produc- tion amount and the extracellular protease activity of the different B. bassiana strains were compared. [ Result] The isolated 9 strains were all B. bassaina （Bals.） Vuillemin, and all strains had high pathogenicity to silkworrfl, but with different pathogenicities. The growth diameter, spore production amount and extracellular protease activity of different B. bassiana strains were also different, and showed correlation with the patheogenicity to silkworms. [ Conclusion] B. bassiana spores production amount and exocellular protease activity had significant positive correlation with their pathogenicity to silkworm.
出处
《安徽农业科学》
CAS
2012年第28期13799-13801,13803,共4页
Journal of Anhui Agricultural Sciences
基金
广西科学研究与技术开发计划项目(10169-08)
珍稀濒危动植物生态与环境保护省部共建教育部重点实验室科研项目(桂科能1001Z014)
关键词
球孢白僵菌
生物学特性
致病力
家蚕
Beauveria bassiana （Bals.） Vuill
Biological characteristics
Pathogenicity
Bombyx mori L.
