摘要
目的:观察心肺复苏(CPR)后不同降温方法对自主循环恢复(ROSC)后兔炎症反应的影响。方法:48只新西兰成年大白兔,采用交流电致颤的方式建立CPR模型,依据降温方式的不同,随机分为常温组(NT)、腹腔内降温组(PC)、体表降温组(SC)和头部局部降温组(LC)。观察不同降温方法对ROSC后血浆TNF-α和IL-6浓度的影响,ROSC后12 h取肝脏组织,Western blotting检测NF-κB p65和NF-κB p50蛋白表达。记录每组动物的存活时间并进行比较。结果:ROSC后4 h、8 h和12 h,PC组血浆TNF-α水平显著低于NT组;至12 h时PC组也显著低于SC组和LC组(P<0.05);ROSC后2 h、4 h、8 h和12 h,PC组血浆IL-6水平明显低于NT组(P<0.05),而与SC、LC组间无显著差异。ROSC后12 h,PC组肝核蛋白内NF-κB p65和p50蛋白表达水平显著低于其它各组(P<0.05),而其它3组之间差异无统计学意义。ROSC后PC组动物的存活率显著高于NT、SC和LC组(P<0.05或P<0.01)。结论:CPR后腹腔降温法可快速诱导亚低温和降低腹腔内温度,抑制肝脏内NF-κB的激活,降低血浆TNF-α和IL-6水平,减轻ROSC后全身炎症反应,延长动物存活时间。
AIM ： To explore the effects of different cooling methods on systemic inflammation after cardiopulmonary resuscitation （CPR） in New Zealand rabbits. METHODS： Ventricular fibrillation in 48 adult New Zealand rabbits was induced by alternating current and was resuscitated after cardiac arrest for 5 min. The rabbits were randomly divided into 4 groups according to the way of cooling methods： normothermia group （NT）, peritoneal cooling group （PC）, surface cooling group （SC） and local cooling group （LC）. The plasma concentrations of tumor necrosis factor α （TNF -α） and interleukin- 6 （IL -6） were measured in each group at different time points after return of spontaneous circulation （ROSC）. The liver tissues were removed 12 h after ROSC, and the levels of NF - κB p65 and NF - κB p50 were tested by Western blotting. The survival time was recorded and compared 96 h after ROSC. RESULTS ： The plasma concentration of TNF - a in PC group was lower than that in NT group 4 h, 8 h and 12 h after ROSC, and was also lower than that in SC group and LC group 12 h after ROSC. The level of IL -6 in PC group was lower than that in NT group 2 h, 4 h, 8 h and 12 h after ROSC, while there was no difference between the other 2 groups. The levels of p65 and p50 in PC group were lower than those in other groups （P 〈 O. 05）, while there was no difference between the other 3 groups. The cumulative survival rate after ROSC in PC group was higher than that in NT group, SC group and LC group. CONCLUSION： The no- vel peritoneal cooling rapidly induces and maintains mild hypothermia, and decreases the peritoneal temperature quickly, thus inhibiting liver NF - κB activation, reducing the releases of TNF - α and IL - 6, subsequently relieving systemic in- flammation after ROSC and prolonging rabbit survival.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2012年第9期1559-1564,共6页
Chinese Journal of Pathophysiology
基金
广东省自然科学基金资助项目(No.S201040003615)
教育部博士点新教师基金资助项目(No.20110171120066)
关键词
心肺复苏
低温
腹腔降温法
炎症
Cardiopulmonary resuscitation
Hypothermia
Peritoneal cooling
Inflammation
