摘要
目的研究岳阳地区汉族人群HLA-A、B、DRB1等位基因多态性的分布特征。方法应用聚合酶链反应-直接测序分型(polymerase chain reaction sequence-based typing,PCR-SBT)高分辨技术,对岳阳地区3 560名无血缘关系的汉族人群HLA-A、-B、-DRB1进行基因分型。结果检出HLA-A、-B、-DRB1等位基因分别有53、89、53种,经统计分析这3个基因座分布均符合Hardy-Weinberg平衡定律。A*0207-B*4601-DRB1*0901和A*3303-B*5801-DRB1*0301单体型是岳阳地区汉族人群最常见单体型。结论岳阳地区汉族人群HLA-A、-B、-DRB1基因分布具有高度的遗传多态性并有其自身分布特点,中国各地常见的基因及罕见基因均有分布。
Objective To analyze the characteristics of the distribution of HLA A, - B and - DRB1 alleles in Han pop- ulation in Yueyang area. Methods HLA- A, - B and - DRB1 genotypes of 3,560 unrelated healthy blood stem cell donors in Yueyang area were determined by polymerase chain reaction sequence- based typing （PCR - SBT） high - resolution tech- nique. The frenquencies of alleles and haplotypes were calculated. Results Totally, 53 HLA- A alleles, 89 HLA- B alleles and 53 HLA- DRB1 alleles were detected. Statistical analysis revealed that the distribution of the three loci all conformed with Hardy- Weinberg equilibrium. A ＊0207 - B ＊＊ 4601 DRB1 ＊ 0901 and A＊3303 - B ＊5801 - DRB1 ＊0301 were the most popular haplotypes in Han population in Yueyang area. Conclusions The distribution of HLA- A, B and - DRB1 genes in Yueyang Hart population has high genetic polymorphism and its own characteristics. All common and rare genotypes in China can be found in this area.
出处
《实用预防医学》
CAS
2012年第9期1307-1310,共4页
Practical Preventive Medicine
