摘要
目的从基因水平调查了中国华南、华北地区人群HLA-DQB1等位基因频率,并研究比较两地区人群HLA-DQB1多态性分布。方法采用深圳益生堂生物企业有限公司研制开发的“HLA-DQB1低分辨率分型基因芯片检测试剂盒”,应用聚合酶链反应-序列特异性引物+序列特异性寡核苷酸探针芯片检测技术,对700名南方地区的中国人和320名北方地区的中国人进行基因分型。结果鉴定了10个HLA-DQB1等位基因,获得了一组准确、科学的统计数据。结论得到了中国华南、华北地区人群HLA-DQB1等位基因频率差异的数据,证明中国人群HLA-DQB1*02,05,0601,0602,0603的分布南北差异有统计学意义(P<0.05),为疾病相关性研究、人文科学研究提供了可靠的遗传学数据。
Objective To research and compare HLA-DQB1 gene frequency（GF） and polymorphism distribution between south and north population of Chinese in China. Methods Combining PCR-sequence specific primers（SSP） and sequence specific ohgonucleotide probe（SSOP） techniques and DNA Microarray Kit for HLA-DQB1 Low Res Genotyping from Shenzhen Yi-Shengtang Biological LTD. Co, was used to type HLA-DQB1 gene polymorphisms of 700 individuals hying in south China and 320 individuals in north China, Results We inspected 10 alleles of HLA-DQB1 and got a series of comprehensive and accurate statistic data. Conclusion It is tested that HLA-DQB1＊ 02,05,0601,0602, 0603 gene frequencies are different obviously（ P 〈 0.05） between south and north Chinese, And those data wiU be useful to kinds of reseach associated with disease relevant and anthropology research,
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
2006年第2期238-239,共2页
Chinese Journal of Medical Genetics
基金
国家863计划项目(2002AA2Z2032)~~
