摘要
利用FeSO4-钼蓝法研究了中草药黄芪(Astragalus membranaceus)粉末、黄芪水提取液对黑曲霉(Aspergillus niger)植酸酶在固体培养基和液体培养基中代谢活性的影响。结果表明,在0.03 g/mL的黄芪粉末和0.03 g/mL的黄芪水提取液作用下,44 h时黑曲霉中植酸酶活性最高。与对照相比,固体培养基中加入黄芪粉末和黄芪水提取液的吸光度分别提高了33.6%和37.8%,液体培养基中加入黄芪粉末和黄芪水提取液的吸光度分别提高了21.0%和28.7%。在最佳培养时间,植酸酶活性均随着黄芪浓度的增大而呈下降趋势,其中加入黄芪粉末的液体培养基变化幅度最大,植酸酶活性浓度0.01 g/mL是浓度0.05g/mL的1.64倍;加入黄芪水提取液的液体培养基中植酸酶活性浓度0.01 g/mL是浓度0.05 g/mL的1.54倍;加入黄芪粉末和黄芪水提取液的固体培养基中植酸酶活性浓度0.01 g/mL分别是浓度0.05 g/mL的1.50和1.42倍。
The effect of traditional Chinese medicine Radix Astragali powder and water extract on Aspergillus niger phytase metabolic activity on solid and liquid culture medium were studied by using FeSO4-molybdenum blue method.The results showed that phytase activity was the highest if treated for 44 hours by 0.03 g/mL of astragalus powder and 0.03 g/mL of water extract.Compared with the control,the absorbance value was improved by 33.6% and 37.8% respectively by adding astragalus powder and astragalus extract water in solid culture medium;however,increase of 21.0% and 28.7% were respectively achieved in liquid culture.In the best culture period,phytase activity increased with the increasing of the concentration of astragalus.There was biggest change range in liquid medium which added astragalus powder.
出处
《湖北农业科学》
北大核心
2012年第17期3786-3788,共3页
Hubei Agricultural Sciences
基金
贵州省中药材现代化产业专项重点项目[黔科合中药专字(2003)52号]
