摘要
以大滨菊的茎段为外植体,对无菌芽诱导、试管苗增殖、生根及移栽技术进行了系统研究。结果表明:大滨菊腋芽诱导的最佳培养基为MS+6-BA 0.5mg/L+NAA 0.05mg/L;最适宜的继代培养基为MS+6-BA 0.3mg/L+NAA 0.03mg/L;生根的最适宜培养基为1/2MS+IBA0.5mg/L。继代周期25d左右,生根需要15d,生根率可达95%以上,移栽成活率达到100%。
The stems of Leucanthemum maximum were used as explants,the aseptic shoots induction,propagation of the plants in vitro,rooting and transplanting were studied.The results showed that the best media for inducing new buts was MS＋6-BA 0.5mg/L＋NAA 0.05mg/L;the best media for propagationg was MS＋6-BA 0.3mg/L＋NAA 0.03mg/L;the best media for rooting was 1/2MS＋IBA 0.5mg/L.The proper subculture cycle was about 25days and the rooting stage took about 15days.The rooting rate was as much as 95%and the survival rate of transplanting was 100%.
出处
《北方园艺》
CAS
北大核心
2012年第19期143-145,共3页
Northern Horticulture
关键词
大滨菊
无菌芽
组织培养
Leucanthemum maximum
aseptic shoots
tissue culture
