摘要
目的研究小檗碱衍生物HB-13对人永生化角质形成细胞(HaCaT细胞)分泌γ干扰素(IFN-γ)的影响,探讨HB-13的抗炎机制。方法采用四甲基偶氮唑蓝法确定HB-13作用于HaCaT细胞的安全浓度;细胞经P物质刺激后,加入不同浓度的HB-13分别孵育12、24、48h,应用酶联免疫吸附法(ELISA)检测培养上清液中IFN-γ的含量,应用实时荧光定量PCR法检测细胞内IFN-γmRNA表达情况。结果 HB-13浓度为2.5、5、10μg/ml对HaCaT细胞毒性作用不显著,并可促进P物质诱导的IFN-γ的分泌。结论促进表皮细胞分泌IFN-γ可能是HB-13在皮肤局部发挥抗炎作用的机制之一。
Objective To study the effect of liB-13 on IFN-y secretion induced by substance P in cultured HaCaT cells, and investigate the anti-inflammatory mechanism of HB-13. Methods The cytotoxicity of HB-13 on HaCaT cells was assessed by MTT method. IFN-γ was induced by substance P in HaCaT cell line, the expression levels of IFN-y in cell supernatant and IFN-y mRNA intra-cellular were detected by ELISA and real-time quantitative PCR at 12, 24 and 48 hours after HaCaT cell incubation with 2.5 μtg/ml, 5 μtg/ml and 10 μtg/ml HB-13. Results 2.5 μtg/ml, 5 μtg/ml and 10 μtg/ml HB-13 almost had no cytotoxicity on HaCaT cell line, but promoted the IFN-γ secretion induced by substance P. Conclusion Anti-inflammatory effects of HB-13 maybe partially accomplished by inducing the production of IFN-γ.
出处
《实用皮肤病学杂志》
2012年第5期265-267,共3页
Journal of Practical Dermatology
基金
甘肃省科技支撑计划基金资助项目(2007GS02454)
