摘要
为揭示不同基因型抗虫棉耐盐性差异的生理机制,指导抗虫棉耐盐品种鉴选和盐碱地棉花生产,以耐盐性差异显著的两类转Bt基因抗虫棉品种——耐盐性强的鲁K536、鲁棉研18优选系,耐盐性弱的新棉33B优选系、鲁K1258——为试验材料,利用不同盐分含量砂培与土培试验相结合,比较研究它们苗期的主要理化指标变化。结果表明,在NaCl胁迫下,耐盐性强的品种苗期叶片内的K+含量显著高于耐盐性弱的品种,Na+含量显著低于耐盐性弱的品种,丙二醛含量也显著低于耐盐性弱的品种,而游离脯氨酸、可溶性糖、类胡萝卜素含量均显著高于耐盐性弱的品种。耐盐性强的品种棉苗的过氧化物酶活性、叶绿素a、b和a+b含量以及净光合速率明显高于耐盐性弱的品种。表明棉花的耐盐性强弱因基因型而异,基因型间的耐盐性差异与丙二醛、脯氨酸、可溶性糖、类胡萝卜素含量和保护酶活性以及盐离子在叶片中累积量的差异有关。
The physiological mechanisms of differences in salt tolerance among four genotypes of insect-resistant cotton were investigated to guide the appraisal of salt-tolerant cultivars and cotton production in saline fields. Sand and soil culture experiments with different salinity levels were conducted with strongly salt-tolerant cultivars （LK536 and the promising selection SCRC18） and weakly salt-tolerant ones （LK1258 and the promising selection 33B） of Bt transgenic cotton. Changes in some physiological and biochemical indices at the seedling stage were compared. The results showed that the comprehensive indices for salt tolerance of LK536 and SCRC18 were significantly higher than those of 33B and LK1258. In seedling leaves of strongly salt-tolerant cultivars, K＋ content was significantly higher, while Na＋ content was significantly lower; malondialdehyde content was also significantly lower, but the contents of flee proline, soluble sugars, and carotenoids were all significantly higher than that in the seedling leaves of weakly salt-tolerant cultivars, respectively, under NaCl stress. The peroxidase activity, contents of chlorophyll a, b and a＋b, and net photosynthetic rate of strongly salt-tolerant cultivars were substantially higher than those of weakly salt-tolerant cultivars at the seedling stage under NaC1 stress. Further analysis indicated that the salt tolerance of cotton varied among genotypes. The variation in salt tolerance among genotypes was related to differences in the contents of malondialdehyde, flee proline, soluble sugars, and carotenoids; in peroxidase activity; and in the accumulation of salt ions in leaves.
出处
《棉花学报》
CSCD
北大核心
2012年第5期406-413,共8页
Cotton Science
基金
国家公益性行业(农业)科研专项(nyhyzx07-005-02)
山东省农业高技术创新基金项目(2007YCX024-03)
关键词
转BT基因抗虫棉
盐胁迫
耐盐性
理化特性
Bt transgenic cotton
NaCl stress
salt tolerance
physiological and biochemical characteristics
