摘要
利用组织培养技术、生化分析的方法，研究了不同盐压力（ＮａＣｌ）对陆地棉愈伤组织增长及蛋白质和氨基酸代谢的影响。结果表明：随着盐浓度不断提高，干重含量增大；蛋白质和氨基酸含量在０．５％～１．Ｏ％ＮａＣｌ浓度时出现峰值；而０．５％ＮａＣｌ浓度附近，愈伤组织停滞后转为迅速增长，其质地由灰色疏松状转变成白色致密状。较低ＮａＣｌ浓度对棉花愈伤组织生长有促进作用，而高ＮａＣｌ浓度对棉花愈伤组织有明显的抑制作用。
The morphology character and protein,amino acid metabolism and the contents ofDW on somatic calli of cotton(G,hirsutum L.)were studied by tissue culture technology andmethod of biochemical analysis. The higher concentration of NaCl contained in the medium,The higher contents of DW of somatic calli,and the maximum value of protein and total AA in0.5%～1.O%NaCl,and calli grow slowly in the begining and then grow rapidly, and thetragile calli with grey color gratually change into the hard calli with light white. The lowerconcentration of NaCl accelerate the growth of calli,and the higher concentraction of NaCl ob-viously suppress the growth of calli。
出处
《棉花学报》
CSCD
北大核心
1994年第1期37-40,共4页
Cotton Science
基金
中国农业科学院院长基金
关键词
盐胁迫
陆地棉
愈伤组织
salt stress,Gossypium hirsutum L. sometic callus
