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两种染色法检测结核分支杆菌的对比观察 被引量:1

Various dyeing in the application of the testing tuberculosis
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摘要 目的比较(intenfised kinyoun,IK)染色法和改良的Wade-Fite染色法检测抗酸菌的临床应用价值。方法采用IK染色法和改良的Wade-Fite染色法对66例病理诊断为慢性肉芽肿性炎,形态符合结核病改变的患者的石蜡组织切片分别进行染色。结果 IK染色法阳性例数为43例,阳性率为65.2%(43/66),其中抗酸分支杆菌L型的阳性率为43.9%(29/66);改良的Wade-Fite染色法阳性例数为24例,阳性率为36.4%(24/66),其中抗酸分支杆菌L型的阳性率为21.2%(14/66),两种方法染色阳性率之间的差异具有统计学意义(P<0.05)。结论 IK染色法检测抗酸菌的阳性率明显高于改良的Wade-Fite染色法。 Objective To compared with IK dyeing and improved wade-rite dyeing in testing Acid fast bacteria in the clinical application value. Methods 66 cases pathological diagnosis for chronic granulomatous inflammation or the form of tuber- culosis were tested by ik dyeing and improved wade-rite dyeing. Results The positive rate in Ik dyeing were 65. 2% (43/66) ,in- cluding the positive rate of acid fast bacteria L were 43.9% (29/66). The positive rate in improved wade-rite dyeing were 36. 4% (24/66). Including the positive rate of the Acid fast bacteria L were 21.2% (14/66). The differences of the positive rate between the two methods dyeing have statistical significance (P 〈 0. 05 ) . Conclusion The positive rate of the Ik dyeing in testing Acid fast bacteria and the acid fast bacteria L were higher than the improved wade-rite dyeing.
作者 杨群 董丹丹
出处 《四川医学》 CAS 2012年第8期1357-1359,共3页 Sichuan Medical Journal
关键词 石蜡切片 抗酸菌L型 IK染色 ararin section acid fast bacteria L-forms Intenflsed kinyoun dyeingp
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