摘要
目的观察肺纤维化小鼠血清中IFN-γ、IL-4、TNF-α的含量、生化指标,探讨地塞米松干预治疗肺纤维化的作用机理。方法昆明小鼠30只随机分为空白组、博莱霉素(BLM)组、地塞米松(DXM)组。采用气管内注入BLM复制肺纤维化模型,造模后第2天使用地塞米松药物干预治疗,第14天采用酶联免疫吸附法测定血清IFN-γ、IL-4、TNF-α及羟脯氨酸(HYP)含量。结果与空白组相比较,BLM组大鼠血清IFN-γ含量降低,IL-4、TNF-α的含量升高(P<0.05),BLM组各时间点Hyp含量有明显增加(P<0.01)。DXM与BLM组Hyp相比较,Hyp含量于第14天和第28天有明显下降(P<0.05)。结论应用地塞米松可以明显减轻大鼠肺纤维化进程,不仅降低Ⅱ型细胞因子(IL-4)含量,而且升高Ⅰ型细胞因子(IFN-γ)及TNF-α含量,从而调整Ⅰ型/Ⅱ型细胞因子平衡及TNF-α表达抗纤维化作用。
Objective To observe the effect of dexamethason on the levels of IFN-γ,IL-4 and TNF-α in serum and explore pathological structure of lung tissue in BLM-induced pulmonary fibrosis rats.Methods Thirty mice were divided randomly into normal,model,and dexamethason groups.Pulmonary fibrosis model was established by injection of bleomycin（BLM） via trachea（5 mg/kg）.After 24 h,rats were given drugs by ig in all groups once a day.On the 14th and 28th day,6 mice were killed and the lungs were collected for observing pathological structure of lung tissue by HE staining.Interleukin-4（IL-4）,interferon gamma（IFN-γ） and tumor necrosis factor-α（TNF-α） in serum and hydroxyproline（HYP）content were detected by enzyme linked immunosorbent assay（ELISA）.Results Compared with the normal group,IFN-γ content was significantly lowered,while IL-4 and TNF-α considerably increased in the model group（P〈0.05）.Compared with the model group,IFN-γ content was significantly elevated（P〈0.05） and IL-4,TNF-α levels significantly decreased（P〈0.05）.The degree of fibrosis was alleviated in Astragalus decoction and Astragalus polysaccharide groups（P〈0.05）.Conclusion Astragalus polysaccharides have protective effect by regulating pulmonary fibrosis in rat Ⅰ/Ⅱ type cytokines balance and TNF-α level,thereby interfering with the occurrence of pulmonary interstitial fibrosis and development.
出处
《实用临床医药杂志》
CAS
2012年第15期17-19,共3页
Journal of Clinical Medicine in Practice
关键词
肺纤维化
地塞米松
细胞因子
羟脯氨酸
pulmonary interstitial fibrosis
dexamethason
cytokine
hydroxyproline
