摘要
目的:建立钩藤总生物碱有效部位质量控制方法。方法:采用酸性染料染色法测定钩藤总生物碱的含量,采用高效液相色谱法测定有效部位中钩藤碱的含量,采用高效液相色谱法建立钩藤总生物碱有效部位的指纹图谱。结果:钩藤总生物碱的含量均在50%以上,钩藤碱在5.75～92μg/mL之间线性关系良好,平均回收率为98.82%,通过指纹图谱相似度评价,不同批次的钩藤总生物碱有效部位的化学成分存在差异。结论:本研究建立的质量控制方法可有效控制钩藤总生物碱有效部位的质量。
Objective：To establish the method for quality control of total alkaloids in Ramulus Uncariae Cum Uncis. Methods：The contents of total alkaloids were determined by acid dye colorimetry,HPLC was used to measure the contents of rhynchophylline and fingerprint of total alkaloids in Romulus Uncariae Cure Uncis. Results：The contents of total alkaloids were higher than 50%,the linear range of rhynchopHylline was 5.75-92 μg/mL,the average re- covery was 98.82%. The chemical composition of different batches total alkaloids in Ramulus Uncariae Cum Uncis was difference by fingerprint similarity evaluation. Conclution：The method can be used for quality control of total alkaloids in Ramulus Uncariae Cum Uncis.
出处
《山东中医药大学学报》
2012年第5期445-448,共4页
Journal of Shandong University of Traditional Chinese Medicine
基金
山东省科技发展计划项目(编号:2008GG2NS02011)
国家科技重大专项重大新药创制
山东省重大新药创制中心建设(编号:2009ZX09301-013)
山东省自然科学基金项目(编号:ZR2010HM017)
山东省教育厅科技计划项目(编号:J105LL05)
国家自然科学基金项目(编号:810727941H2708)
关键词
钩藤总生物碱
钩藤碱
指纹图谱
total alkaloids in Ramulus Urwariae Cure Uncis
rhynchophylline
fingerprint
