摘要
【目的】为研究2,4-表油菜素内酯(2,4-epibrassinolide,EBR)和脱落酸(abscisic acid,ABA)处理对葡萄花色苷合成与可溶性固形物含量、果皮苯丙氨酸解氨酶(PAL)和类黄酮糖基转移酶(UFGT)活性以及果实内源ABA含量的影响,探索油菜素内酯调控葡萄果实成熟及花色苷合成的机理。【方法】以酿酒葡萄‘赤霞珠’(Cabernet Sauvignon)和‘烟73’(Yan 73)为试材,在葡萄转色前分别用0.1、0.4、0.8 mg.L-1EBR,1mg.L-1Brz(brassinazole,BR生物合成抑制剂)和200 mg.L-1ABA,均匀喷施于葡萄果实,在葡萄成熟过程中测定葡萄果皮花色苷含量及PAL和UFGT酶活性,同时测定果实ABA和可溶性固形物含量。【结果】在果实着色初期,‘赤霞珠’和‘烟73’葡萄果皮PAL和UFGT活性及果实内源ABA含量均逐渐升高,当果实接近成熟花色苷含量基本稳定时ABA含量开始降低。与对照相比,0.4mg.L-1EBR和200 mg.L-1ABA处理显著增加了果实内源ABA含量,提高了果皮PAL和UFGT活性,促进了果皮花色苷的合成和果实可溶性固形物的积累。0.1 mg.L-1和0.8 mg.L-1EBR处理总体增加了果实内源ABA含量,促进了花色苷的合成和可溶性固形物的积累,并提高了UFGT和PAL酶活性,但比0.4 mg.L-1EBR处理提高的幅度小,且差异显著。1 mg.L-1Brz处理使果实ABA的合成推迟,果实可溶性固形物含量、果皮PAL和UFGT活性以及花色苷含量均低于对照,但差异不显著。【结论】外源EBR和ABA处理促进了葡萄成熟和花色苷合成,在不同浓度EBR处理中,以0.4 mg.L-1处理效果较好;内源ABA可能参与了EBR对葡萄成熟和花色苷合成的调控。
[Objective]In order to explore the mechanism of regulation of ripening and anthocyanin biosyn- thesis in grape berry by brassinosteroids, the effects of 2,4-epibrassinolide （EBR） and abscisie acid （A= BA） on anthoeyanin biosynthesis, the content of soluble solids and endogenous ABA, the enzyme activities of phenylalanine ammonia-lyase （PAL） and UDP-glucose-flavonoid-3-O- glycosyhransferase （UFGT） in grape skin were studied. [Method]Different concentrations of exogenous 2,4-epibrassinolide （0.1, 0.4, 0.8 mg·L^（-1））, brassinazole （Brz, 1 mg·L^（-1）, an inhibitor of BR biosynthesis） and abseisic acid （ABA, 200 mg·L^（-1））were respectively applied to the berries of wine grape, Cabernet Sauvignon and Yan73, before veraison. The anthocyanin content and the activities of phenylalanine ammonia- lyase （PAL）, UDP-glucose- flavonoid-3-O- glycosyltransferase （UFGT） in grape skin were analyzed. The ABA production and the soluble solids were also studied. [Result]The results demonstrated that the activities of PAL and UFGT in berry skin and the content of endogenous ABA in berry increased gradually at the beginning of veraison , and the ABA content decreased when berry was approaching maturity and the content
出处
《果树学报》
CAS
CSCD
北大核心
2012年第5期830-836,共7页
Journal of Fruit Science
基金
现代农业产业技术体系建设专项资金项目(CARS-30)
陕西省自然基金项目(2011JM3004)
西北农林科技大学基本科研业务费专项资金项目(QN2009059)
