摘要
研究了表油菜素内酯（ｅｐｉ－ＢＲ）对拟南芥细胞体外分化的影响．表明ｅｐｉ－ＢＲ不仅能促进愈伤组织的增殖，而且还能有效地诱导愈伤组织转绿，继而分化绿芽和长成小植株，其诱导频率高达７０％以上。电镜观察表明，ｅｐｉ－ＢＲ诱导的转绿细胞中的叶绿体发育正常。
Arabidopsis thaliana have been usedto investigate the role of epibrassinolide(epi-BR) in cell differentiation and regeneration in vitro. The results showthat calli cultured on MS medium supplemented with 0.05, 0.5 or 5.0 mg/L epi-BR formed much green buds andshoots. Supplementing the culturemedium with epi-BR and 0.1mg/L KTinduced the greening of callus and budformation, but KT did not induce this.differentiation alone. In addition, electron microscopic examination showsthat normal chloroplasts were containedin cell of green Callus cultured on medium with epi-BR.
关键词
拟南芥
表油菜素内脂
愈伤组织
细胞分化
epibrassinolide (epi-BR ), Arabidopsis thaliana, callus, cell differentiation
