摘要
目的探讨结核性胸膜炎患者胸腔积液中水通道蛋白-1(APQ-1)表达水平及其与肿瘤坏死因子(TNF-α)、白细胞介素-8(IL-8)的相关性。方法采用ELISA双抗体夹心法测定58例结核性胸腔积液、25例恶性胸腔积液和15例胸腔漏出液中AQP-1、TNF-α和IL-8的含量。结核性胸膜炎患者于入院时和抗结核治疗第1、3、5天留取胸腔积液检测AQP-1、TNF-α和IL-8的含量。结果治疗前结核性胸腔积液患者的AQP-1、TNF-α和IL-8水平均显著高于恶性胸腔积液和漏出液患者(P<0.05)。结核性胸腔积液AQP-1水平与胸腔积液中TNF-α和IL-8水平呈正相关(r分别为0.415、0.397,P<0.01)。结核性胸膜炎组患者在规则治疗第1、3、5天时,胸腔积液AQP-1的含量逐渐下降[(28.5±8.4)ng/ml、(21.6±7.7)ng/ml、(19.6±7.2)ng/ml],与治疗前比较,差异有统计学意义(P<0.05)。结论结核性胸膜炎患者胸腔积液AQP-1水平升高,其与TNF-α和IL-8共同参与了结核性胸腔积液的形成。
Objective To explore expression level of aquaporin-1 （AQP-1） and its correlation with TNF-a and IL-8 in tuberculous pleural effusion. Methods Levels of AQP-1,TNF-a and IL-8 were measured by enzyme linked immunosorbent assay in pleural effusion of 58 patients with tuberculous pleural effusion, 25 patients with malignant pleural effusion and 15 patients with transudate. Levels of AQP-1, TNF-a and IL-8 were measured in tuberculous pleural effusion with patients on admission and anti-tuberculous therapy for 1,3,5th days. Results The levels of AQP-1, TNF-a and IL-8 before treatment in tuberculous pleural effusion were markedly higher than those in malignant effusion and transudate （P 〈 0.05）. The levels of AQP-1 in tuberculous pleural effusion had a positive relation with TNF-a and IL-8（r value was 0.415 and 0.397 respectively, P 〈0.01）. The levels of AQP-1 in tuberculous pleural effusions decreased in the 1 d, 3 d and 5 d after anti-tuberculosis treatment [（36.4±10.2） ng/ml, （28.5±8.4） ng/ml, （21.6±7.7） ng/ml, （19.6±7.2） ng/ml, P 〈 0.05]. Conclusions The levels of AQP-1 in tuberculous pleural effusions increase. AQP-1,TNF-a 和 IL-8 play a role in the formation of tuberculous pleural effusions.
出处
《北京医学》
CAS
2012年第9期801-803,共3页
Beijing Medical Journal
