摘要
目的:建立清热降糖颗粒的质量标准。方法:采用薄层色谱法对制剂中黄连、葛根及黄芩进行定性鉴别,以HPLC法对制剂中黄芩苷进行定量测定。结果:黄连、葛根及黄芩薄层色谱斑点清晰,阴性对照无干扰。黄芩苷进样量在0.069 28～1.385 6μg范围内与色谱峰面积积分值呈良好线性关系(r=0.999 7),平均回收率为99.21%,RSD为0.40%(n=5)。测得3批样品中的黄芩苷含量分别为30.46、32.68和31.54 mg.g-1。结论:所建标准可用于清热降糖颗粒的质量控制。
Objective：To establish the quality standard of Qing-Re-Jiang-Tang Granules.Methods：The thin layer chromatography（TLC） method was used to identify Coptidis Rhizoma,Puerariae Lobatae Radix and Scutellariae Radix in the recipe qualitatively.The content of baicalin in the recipe was determined by HPLC.Results：The TLC spots were clear without the interference from negative sample.Baicalin showed a good linear relationship at a range of 0.06928~1.3856 μg（r=0.999 7）.The average recovery was 99.21%,and RSD was 0.40%（n=5）.The content of baicalin in 3 batches of samples was 30.46,32.68 and 31.54 mg ·g-1,respectively.Conclusion：The established standard can be used for the quality control of Qing-Re-Jiang-Tang Granules.
出处
《药学进展》
CAS
2012年第8期373-376,共4页
Progress in Pharmaceutical Sciences
关键词
清热降糖颗粒
质量标准
黄芩苷
薄层色谱
高效液相色谱
Qing-Re-Jiang-Tang Granules
quality standard
baicalin
thin layer chromatography
HPLC
