摘要
从受有机磷农药长期污染的土壤中通过富集培养，分离筛选到一株降解乐果活性较高的曲霉Ｚ_５８菌株，研究了该菌株的最适产酶条件。培养温度３０℃，培养起始ｐＨ７．０，培养时间９６ｈ；酶反应的最适温度和ｐＨ分别为４５℃和７．２，在４０℃以下及６０～９．０范围内稳定，主要作用底物为有机磷农药。
Aspergillus sp. Z_58, which was isolated from samples contaminated by organophosphorus pesticides, produced dimethoate-degrading enzymes. The optimal temperature, initial pH and culture time for enzyme production were 30℃, 7.0 and 96 hours, respectively. The optimal pH and temperature for enzyme activity was 7.2 and 45℃, respectively. The enzymes were stable among the range of pH 6.0-9.0 and below 40℃. It showed strong activity towards organophosphorus pesticides which were tested.
出处
《微生物学通报》
CAS
CSCD
北大核心
2000年第3期162-165,共4页
Microbiology China
关键词
曲霉
有机磷农药降解酶
产酶条件
酶学性质
Aspergillus sp., Organophosphorus pesticides-degrading enzyme, Enzyme formation and properties
