摘要
建立了高效液相色谱法对土壤中除草剂精喹禾灵残留的测定方法,并研究了其微生物降解特性。结果表明,采用C18柱和DAD检测器,以甲醇和0.2%磷酸水溶液(274∶,V/V)为流动相,在波长234 nm进行检测,土壤中精喹禾灵的回收率为88.4%～90.4%,相对标准偏差低于6.75%。未灭菌土壤中精喹禾灵的降解速率比灭菌土壤中快,接种优势微生物后精喹禾灵的降解速率明显加快,表明微生物是土壤中精喹禾灵降解的主要因素。
Determination and microbial degradation of quizalofop-p-ethyl in soil was studied using high performance liquid chromatography. The samples were separated on C18 column, with methanol-water （27：4,V/V） as mobile phase. The samples were detected at 234 nm with DAD detector. The recovery for the fortified samples with known amounts of Quizalofop-p-ethyl were found to be in a range of 88.4%-90.4% in soil with relative standard deviation of less than 6.75%. The method was proved to be accurate, rapid and reproducible. Microbial degradation of quizalofop-p-ethyl in soil media was investigated using bacteria, Bacillus megaterium, those were separated from whale field in Jiaxing. The biodegradation experiment was carried out under the condition of 29 ℃. The biodegradation rate of quizalofop-p-ethyl in bacteriacontaining soil was faster than that in the media without bacteria, indicating that bacteria are the main factor for quizalofop-p-ethyl degradation in the tested soil. The degradation rates of quizalofop-p-ethyl in a sterilized soil and bacteria-containing soil in ten days reached at 35.1% and 68.3%, respectively. The degradation rate of quizalofop-p-ethyl in the soil containing incubated and purified bacteria in ten days could reach at 89.3%. The results indicted that the bacteria separated from the soil samples can degrade quizalofop-p-ethyl with high efficiency.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2006年第B09期742-745,共4页
Journal of Agro-Environment Science
关键词
精喹禾灵
高效液相色谱法
微生物降解
quizalofop-p-ethyl
high performance liquid chromatography
microbial degradation
