摘要
2010年从佛山市某专业户送检的雏鹅肝组织中分离到1株鹅细小病毒(命名为SS/10株),并对其生物学特性进行了研究。该毒株的番鸭胚半数致死量为10-4.6/0.3 mL,动物回归试验显示其对10日龄雏鹅没有致死性,从组织切片观察肠、肝脏、肾脏和脑组织均有明显的病理变化。对该毒株测序分析结果发现,其VP1、VP2和VP3基因与GenBank收录的番鸭分离株DY株的同源性较其他参考毒株(鹅分离株)的同源性低,NS1和NS2基因的同源性没有这种差异;其VP3基因与82-0321V,VG32/1和标准株B株在同一分支上,与82-0321V亲缘关系最近,与番鸭细小病毒GD株亲缘关系最远;其VP3基因与B株相比少了505-507位的糖基化位点NRT。
A strain of goose parvovirus（SS/10） was isolated from gosling hepar and biological characteristics of the virus were studied.The result indicated that the ELD50 to muscovy duck embryos was 10-4.6/0.3 mL and no goslings were dead after inoculation,but pathological change by tissue section was evident.Sequence analysis showed that the homology with DY strain was lower than other reference strains in VP1,VP2 and VP3 genes respectively and there was no difference in NS1 and NS2 genes;VP3 gene belonged to a branch with 82-0321V,VG32/1 and B strains published in GenBank.It had closest genetic relationship with 82-0321V and farthest genetic relationship with muscovy duck parvovirus GD strain.Glycosylation sites analysis showed that VP3 had one less glycosylation site than B.
出处
《中国畜牧兽医》
CAS
北大核心
2012年第8期56-60,共5页
China Animal Husbandry & Veterinary Medicine
基金
农业科技成果转化资金项目(2011GB2E000005)
关键词
鹅细小病毒
分离鉴定
组织切片
序列分析
生物学特性
goose parvovirus
isolation and identification
tissue section
sequence analysis
biological characteristics
