摘要
目的:修订完善牛黄解毒丸现行标准中黄芩苷的含量测定方法。方法:参照《中国药典》2010年版黄芩苷含量测定的方法,优化了测定方法。采用Welch Matenals C18(4.6 mm×200 mm,5μm)色谱柱,甲醇-水-磷酸(45∶55∶0.2)为流动相,流量1.0 mL.min-1。结果:以进样量为横坐标,峰面积为纵坐标,建立回归方程Y=2 105.3X-3.577 7,r=0.999 8,黄芩苷在0.028 46～0.853 8μg范围内呈良好的线性关系,平均回收率为103.2%(n=6),RSD为1.29%。结论:修订了的含量测定方法科学、合理,重复性好,可用于该药的质量控制。
Objective： Revising and improving the existing standard of content determination of baicalin in Nihuang Jiedu Pills . Methods：Refer to the Chinese Pharmacopoeia 2010 edition of baicalin content determination , improve the method. HPLC separation was achieved on welch matenals C la （4. 6 mm × 250 mm,5 μm）. The mobile phase was methanol-water-phosphoric acid （45：55：0. 2） at the flow rate of 1.0 mL .min^-1. Results： The regression equation Y=2 105. 3X -3. 577 7, r =0. 999 8; results showed that baicalin in the range of 0. 028 46 -0. 853 8 μg was in good linear relationship. The average recovery was 103.2% （n =6） , and RSD was 1.29%. Conclusion： The revised method for the determination of baicalin in Nihuang Jiedu Pills was scientific, rational, feasible, in good reproducibility, and can be used for quality control of Niuhuang Jiedu Pills
出处
《中国药品标准》
CAS
2012年第4期275-278,共4页
Drug Standards of China
