摘要
目的:优选消食导滞凝胶的水提工艺条件。方法:以栀子苷为指标,HPLC测定含量,Agilent XDB-C18色谱柱(4.6 mm×150 mm,5μm),流动相乙腈-水(10∶90),检测波长238 nm,流速1.0 mL.min-1,选取加水倍数、提取时间、提取次数为考察因素,采用L9(34)正交试验优选消食导滞凝胶的提取工艺。结果:最佳水提取工艺为加6倍量水提取2次,每次1h。结论:该优选工艺流程操作简单,生产成本低,适合于工业生产的需要。
Objective： To optimize water extraction technology of Xiaoshi Daozhi gel. Method： With the content of geniposide as index which was determined by HPLC, Agilent XDB-C18 column （4.6 mm × 250 mm, 5 μm）, acetonitrile-water （10： 90） as mobile phase with flow rate of 1.0 mL · min-1, UV detection wavelength was 238 nm, orthogonai test was used to optimize extraction technology of Xiaoshi Daozhi gel with water volume, extraction times and extraction time as factors. Result： Optimal water extraction technology was as follows： extracted 2 times with 6 times the amount of water, 1 h per time. Conclusion： This optimized technology was simple with low cost, it was suitable for industrial production.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第17期42-43,共2页
Chinese Journal of Experimental Traditional Medical Formulae
