摘要
目的:优选蛇床子挥发油羟丙基-β-环糊精(HP-β-CD)包合物的制备工艺。方法:以挥发油利用率和包合物得率为考察指标,采用正交试验法对蛇床子挥发油HP-β-CD包合物的工艺进行优化。采用UV技术、显微鉴定、GC-MS技术对包合物进行质量控制研究。结果:最佳包合工艺条件为蛇床子挥发油与HP-β-CD的体积质量比1∶8,包合时间2.0 h,包合温度40℃。采用紫外光谱法验证包合前后紫外吸收图谱基本一致,显微鉴定表明油已被包合,GC-MS验证了包合前后挥发油中各成分基本未发生变化。结论:该提取、包合工艺合理可行,为临床制剂的合理使用和包合物开发提供实验依据。
Objective： To optimize preparation technology of inclusion compound of volatile oil from Cnidium monnieri with hydroxypropyl-β-cyclodextrin （HP-β-CD）. Method ： With utilization rate of volatile oil and yield of inclusion compound as indexes, inclusion compound of volatile oil from C. monnieri with HP-β-CD was optimized by orthogonal design. Quality control of inclusion compound was studied by UV technology, microscopic identification and GC-MS analysis. Result： Optimum inclusion technology was： volatile oil to HP-β-CD 1：8 （mL·g-l） , inclusion time 2.0 h, inlusion temperature 40 ℃. It verified that UV absorption spectrum was the same before and after inclusion, volatile oil had been inclusion by microscopic identification, GC-MS verified that composition in volatile oil was essentially unchanged before and after inclusion. Conclusion： Both of extraction and inclusion technology were reasonable and feasible, it could provide experimental basis for rational use of clinical formulation and development of inclusion.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第17期28-31,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
蛇床子挥发油
羟丙基-Β-环糊精
包合物
volatile oil from Cnidium monnieri
Hydroxypropyl-β-cyclodextrin
inclusion compound
