摘要
目的:优化阿里红中总三萜酸的提取工艺,并建立其含量测定方法。方法:以齐墩果酸为对照品,采用分光光度法测定三萜酸含量;采用单因素试验考察提取溶剂种类及浓度、料液比、温度、提取时间及提取次数对总三萜酸得率的影响,在此基础上,采用L9(34)正交试验优选阿里红中总三萜酸提取工艺。结果:最佳提取工艺为30倍量90%乙醇于60℃超声提取3次,每次30 min。在此条件下,阿里红中总三萜酸质量分数35.1%。各因素影响次序为提取温度>液料比>提取时间>乙醇体积分数。结论:阿里红中三萜酸含量很高,该优选工艺稳定可行,可推广于大生产。
Objective： To optimize extraction technology of total tritepenoidic acid from Fomes officinalis and establish its content determination method. Method： The content of total tritepenoidic acid was determined by UV-Vis with oleanic acid as reference; Effects of kind and concentration of extraction solvent, solid-liquid ratio, temperature, extraction time .and extraction times on yield of total tritepenoidic acid were investigated by single factor test, basis on this, extraction technology was optimized by orthogonal test. Result： Optimum conditions of ultrasonics extraction were as follow： extracted 3 times at 60 ℃ with 30 times the amount of 90% ethanol, 30 min each time. Under these conditions, effect of factors on yield was in order of extraction temperature 〉 solid-liquid ratio 〉 extraction time 〉 the concentration of ethanol, yield of total tritepenoidic acid from F. Officinalis was 35. 1%. Conclusion： The content of triterpenoidic acid from F. officinalis was very high, this optimized technology was stable and feasible, which could be extended to large-scale production.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第17期24-27,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
新疆大学校院联合项目(XY110115)
新疆维吾尔自治区自然科学基金项目(2011211A015)
关键词
阿里红
总三萜酸
正交试验
超声提取
Fomes officinalis
total tritepenoidic acid
orthogonal experiment
ultrasonic extraction
