摘要
目的 :建立高效液相色谱方法同时检测人血清中全反式及 13 -顺式维甲酸浓度。方法 :色谱柱 :μBondapakC18(3 9mm× 30 0mm) ,流动相 :甲醇 -醋酸铵缓冲液 (85∶15 ) ,流速 :0 8mL·min-1,紫外检测波长 :340nm ,柱温 :2 2℃ ,血清样品经乙醚 2次提取 ,按内标法定量 ,内标物选用对二甲氨基苯甲醛。结果 :全反式及 13-顺式维甲酸的线性范围分别在 0 8～ 112 0 μg·L-1和 0 82～ 1312 μg·L-1,最低检测浓度均为 0 6μg·L-1。全反式维甲酸测定的平均回收率为 98 86 %～ 10 5 2 % ,日内、日间精密度RSD为 0 84%～ 5 5 % ,13-顺式维甲酸测定的平均回收率为 10 1 6 %～ 10 1 8% ,日内、日间精密度RSD为 1 4%～ 5 7%。结论 :本方法灵敏、准确 ,样品处理简便易行 。
Objective:To develop an isocratic HPLC for the simultaneous determination of all- trans -and 13- cis -retinoic acid(ATRA,13-cRA)in human serum.Methods:The serum sample(1 mL)was extracted with 5 mL of ether for twice.The extract was analyzed on an μBondapak C 18 (3 9 mm×300 mm)column with a mobile phase consisting of 85 Vols of methanol and 15 Vols of NH 4Ac buffer(pH 6 0)at 22 ℃.The flow rate was 0 8 mL·min -1 .The retinoids were detected at 340 nm and quantitated by internal standard method,DMAB was used as an internal standard.Results:The linearity of method was in the range of 0 8 to 1 120 μg·L -1 for ATRA and 0 82 to 1 312 μg·L -1 for 13-cRA.The limit of determination was 0 6 μg·L -1 in serum for both ATRA and 13-cRA.Average recoveries were 98 86%～105 2% for ATRA ,101 6%～101 8% for 13-cRA.Within-day and between-day percision of determination were 0 84%～5 5% for ATRA and 1 4%～5 7% for 13-cRA.Conclusion:This method is sensitive,accurate and good enough to be utilized for the investigation of the clinical pharmacokinetics of ATRA.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2000年第4期226-227,共2页
Chinese Journal of Pharmaceutical Analysis
关键词
全反式维甲酸
顺式维甲酸
血药浓度
HPLC
all- trans -retinoic acid,13-cis -retinoic acid,serum drug concentration,HPLC
