摘要
目的 :研究应激免疫抑制蛋白 (immunosuppressiveproteinofstress,ISPS)的细胞来源。 方法 :免疫荧光染色后 ,运用流式细胞仪和激光扫描共焦显微镜两种技术对ISPS进行定性和定量研究。结果 :流式细胞仪结果发现无论在正常还是应激条件下 ,ISPS均在T淋巴细胞上表达 ,且应激后ISPS表达量在T淋巴细胞显著升高至正常时的 3倍。在激光扫描共焦显微镜下 ,直接观察到分离纯化的T淋巴细胞中有ISPS阳性细胞存在 ,且发现T细胞的两类亚群均具有产生ISPS的功能。结论
Objective: To identify the cell source of immunosuppressive protein of stress(ISPS). Methods: Fluorescence activated cell sorter (FACS) and confocal laser microscope were used to analyze ISPS in purified T cells. Results: FACS analysis showed ISPS increased significantly under stress condition in T lymphocytes. ISPS positive cells were also observed among the purified T lymphocytes through confocal laser microscope. We also found that both subtypes of T lymphocytes can produce ISPS. Conclusion: T lymphocytes can produce ISPS.
出处
《北京医科大学学报》
CSCD
2000年第4期332-333,共2页
Journal of Peking University(Health Sciences)
关键词
应激免疫抑制蛋白
T细胞
分泌
Immunosuppressive protein of stress
T- lymphocytes/secret
T- lymphocyte subsets/secret
