摘要
目的：研究寒冷与耗竭性游泳两种不同方式的应激对束缚应激免疫抑制蛋白产生的影响。方法：应激后小鼠血清影响淋巴细胞转化和高效液相法测定血清中免疫抑制蛋白的分子量。结果：小鼠在耗竭性游泳后血清中产生免疫抑制蛋白，分子量为1．55×105；寒冷应激后小鼠血清中不产生免疫抑制蛋白。结论：束缚应激免疫抑制蛋白的产生具有普遍意义。
Obective: To study different effects on generation of immune suppresso under the stress ofexhausting swimming and cold. Methods: Lymphocyte proliferation and HPLC gel filtration methch wereperformed. Results: The mice were treated with the stress of exhausting swimming for 12 h and the serumfrom stressed mice was found to be able to suppress normal lymphocyte (from unstressed mice) proliferationinducedby ConA. To further determine the molecular weight of suppressive factor in the serum from stressedanimal gel filtration HPLC was used and the fractions which suppressed lymphocyte proliferation wereidentified as 1. 55×1O5 which was quite similar to the suppressive protein(1. 55×l05 ) induced by restraintstress in mice and rats. However, the serum from the mice treated with cold(4℃, 12 h)had no suppressiveeffect on normal lpophocyte proliferation induced by ConA. Conclusion: The suppressive protein can beinduced by various kinds of stress but not all kinds of the stress.
出处
《北京医科大学学报》
CSCD
1997年第4期323-325,共3页
Journal of Peking University(Health Sciences)
关键词
应激
免疫抑制
寒冷
Stress/immunal Lymphocyte transformation Immunosuppression
