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胡萝卜软腐欧文氏菌中信号分子合成酶expI基因的克隆、表达及其分析 被引量：3

Cloning,expression and analysis of expI gene of Erwinia carotovorasubsp.carotovora
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摘要 用PCR方法从胡萝卜软腐欧文氏菌的基因组DNA中扩增出信号分子合成酶expI基因,将其克隆到大肠杆菌表达载体pET-28α(+)上,转化大肠杆菌BL21(DE3),获得高效表达expI基因的重组大肠杆菌BL21(pET28α-expI)。重组菌经IPTG诱导表达,SDS-PAGE检测表达蛋白相对分子质量约为24.8 kD,与预期分子量相符。经薄层层析和高效液相色谱分析发现该重组菌产生的信号分子种类为N-3-羰基己酰高丝氨酸内酯和N-己酰高丝氨酸内酯与胡萝卜软腐欧文氏菌产生的一致。 An expl gene was amplified with PCR from the genomic DNA of Erwinia carotovora subsp, carotovora and was cloned into the expression vector pET-28 a （ ＋ ）. The hybrid plasmid was transformed into Escherichia coli B[21 （ DE3 ）. A recombinant strain harbouring expI gene was obtained. An expression product with a molecular of 24. 8kD weight was detected by SDS-PAGE after induction by IPTG. TLC and HPLC analysis revealed that the recombinant strain produced similar types of quorum sensing signals with Erwinia carotovora subsp carotorave.
作者 黄媛媛 边子睿 宋水山
机构地区 河北省科学院生物研究所 河北省主要农作物病害微生物控制工程技术研究中心
出处 《生物学杂志》 CAS CSCD 2012年第4期60-64,共5页 Journal of Biology
基金 国家重点基础研究发展计划(2009CB126000) 河北省自然科学基金(C2006000707) 河北省重点基础研究计划项目(08965506D)
关键词 胡萝卜软腐欧文氏菌 expI基因 克隆 薄层层析 高效液相色谱 Erwinia carotovora subsp. Carotovora expl gene cloning TLC HPLC
分类号 Q786 [生物学—分子生物学]
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参考文献15

  • 1罗利龙,娄瑞娟,邱健,宋水山.细菌群体感应及其干扰策略的研究进展[J].生物学杂志,2010,27(6):79-82. 被引量：4
  • 2Neslson K H, Hastinsg J W. Bacterial bioluminescence: its control and ecological significance [ J]. Microbiol Rev, 1979, 43 : 496 - 518.
  • 3Schauder S, Bassler B L. The languages of bacteria [ J ]. Genes Dev, 2001, 15(2) :1468 - 1480.
  • 4Randy O C, Mignel M T, Jose L B. N-acyl-L-homoserine lactones: a class of bacterial quorum-sensing signals alter post-embryonic root de- velopment in Arabidopsis thaliana [ J ]. Plant Cell Environ, 2008, 31 : 1497 - 1509.
  • 5Mathesius U, Mulders S, Gao M S, et al. Extensive and specific re- sponses of a eukaryote to bacterial quorum-sensing signals ~ J ]. Prog Natl Acad Sci USA, 2003, 100:1444 - 1449.
  • 6Rad U V, Klein I, Dobrev P I, et al. Response of Arabidopsis thali- ana to N-hexanoyl-dl-homoserine lactone, a bacterial quorum sensing molecule produced in the rhizosphere[ J]. Planta, 2008, 229:73 -85.
  • 7黄媛媛,邱健,马宏,苏浴源,宋聪,宋水山.N-己酰高丝氨酸内酯对马铃薯生长及抗软腐病能力的影响[J].安徽农业科学,2010,38(2):559-561. 被引量：3
  • 8边子睿,刘方,张霞,张哲,宋水山.GCR1和GCR2在N-酰基高丝氨酸内酯调节拟南芥根生长中的作用[J].植物生理学报,2011,47(2):175-180. 被引量：8
  • 9Schuhegger R, Ihring A, Gantner S, et al. Induction of systemic re- sistance in tomato by N-acyl-L-honoserine lactone-producing rhizo- sphere bacteria[ J]. Plant Cell Environ, 2006, 29:909 -918.
  • 10Pang Y, Liu X, Ma Y, et al. Induction of systemic resistance, root colonization and biocontrol activities of the rhizospheric strain of Ser- ratia plymuthica are dependent on N-acyl homoserine lactones [ J]. European Journal of Plant Pathology, 2009, 124 : 261 - 268.

二级参考文献111

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同被引文献22

  • 1陈峰.微生物的群体感应信号分子(综述)[J].上海农业学报,2005,21(1):98-102. 被引量：8
  • 2刘玉升,叶保华,吕飞,郑继法.豆天蛾幼虫肠道细菌分离及初步鉴定研究[J].山东农业大学学报（自然科学版）,2006,37(3):345-348. 被引量：10
  • 3Whitchead N A, Banard A M, Slater H, et al. Quorum-sensing in Gram- negative bacteria[J]. FEMS Microbiol Lett, 2001, 25:364-404.
  • 4Sun W Q, Cao J G, Teng K, et al. Biosynthesis of poly-3-hydroxybutyrate in the luminescent bacteriu, Vibrio harvey/, and regulation by the lux au- toinducer, N- (3-hydroxybutanoyl) homoserine lactane [ J ]. J Biol Chem, 1994, 269:20785-20790.
  • 5Hoffman N, Rehm B H. Regulation of polyhydroxyalkanoate biosynthesis in pseudomonas putida and pseudomonas aeruginosa [ J ]. FEMS Microbiol Lett, 2004, 237 : 1 - 7.
  • 6Hoffman N, Rehm B H. Nitrogen-dependent regulation of medium-chain length polyhydroxyalkanoate biosynthesis genes inpseudomonaz[J]. Bio- technol Lett, 2005, 27:279 - 282.
  • 7Lyndal S T, Jeremy S W, Scott A R, et al. The alternative sigma factor RpoN regulates the quorum sensing gene rhll in pseudomonas aeruginosa [J]. FEMS Microbiol Lett, 2003, 220:187 -195.
  • 8I Brazilian National Genome Project Consortium. The complete genome se- quence of Chromobacterium violaceum reveals remarkable and exploitable bacterial adaptability[ J]. PNAS, 2003, 100(20) : 11660 - 11665.
  • 9Poblete-castro I, Escapa I F, Jager C, et al. The metabolic response of P. putida KT2440 producing high levels of polyhydroxyalkanoate under single-and multiple-nutrient-limited growth: highlights from a multi-level omics approach[J]. Microb Cell Fact, 2012, 11:34.
  • 10]Alexander S, EI-Mdhdi D, Robert H, et al. Synthesis and production of poly(3-hydroxyvaleric acid) homopolyester by Chromobacterium violace- um [ J ]. Appl Microbiol Biot , 1993, 39:443 - 449.

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生物学杂志
2012年 第4期

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