摘要
[目的]研究细菌个体间通讯信号N己-酰高丝氨酸内酯(C6-HSL)是否可以影响植物的生长和抗病能力。[方法]以马铃薯脱毒苗为试验材料,C6-HSL为诱导剂研究了经不同浓度C6-HSL处理后,马铃薯生长、抵抗胡萝卜软腐欧文氏菌浸染的情况,特别研究了C6-HSL对植株标志性防御酶活性和H2O2含量的影响。[结果]不同浓度的C6-HSL能明显抑制马铃薯的出根率和生根数,而对株高、茎节数及叶片大小没有影响;植物标志性防御酶类中POD和SOD的活性以及H2O2的产生量经C6-HSL诱导后显著提高,而PAL和PPO的活性则没有明显变化;抗病试验中,经C6-HSL诱导的马铃薯植株能有效抑制胡萝卜软腐欧文氏菌的侵染,发病率明显低于对照组。[结论]细菌AHL有可能成为一种新的植物抗病激活剂。
[ Objective ] The aim was to study whether C6-HSL can affect plant growth and disease resistance ability. [ Method ] With potato virus-free plantlets as test materials and C6-HSL as revulsant, the potato＇ s growth and the ability of resistance against Erwinia carotovora subp. carotovora were tested after treated with different concentration of C6-HSL. The effect of C6-HSL on plant landmark defense enzyme activities and H202 concentration in potato leaf were measured especially. [ Result] The results revealed that different concentration of C6-HSL could obviously inhibit the growth rate of root and the number of roots, but have no effects on plant height, number of nodes and leaf size. The POD and SOD activities and the concentration of H202 showed a significantly increased when the potato implants were pretreated with C6-HSL, how- ever, no effect of C6-HSL pretreatment on PAL and PPO activities were found. The ability of resistance aginst Erwinia carotovora subp. caroto- vora was signifinanfly improved after treated by C6-HSL. Incidence was obviously lower than the control group. [ Conclusion] Bacteria AHL could be used as a new kind of plant disease resistance activator.
出处
《安徽农业科学》
CAS
北大核心
2010年第2期559-561,共3页
Journal of Anhui Agricultural Sciences
基金
河北省自然科学基金项目(C2006000707)
