摘要
采用阻断ELISA方法对来自天峻县的327份牦牛血清进行了牛BVDV特异性抗体的检测,共检出231份血清阳性,血清阳性率为70.64%。其中:在135份野牦牛血清中检出110份阳性血清,阳性率为81.48%;在192份家牦牛血清中检出阳性121份,阳性率为63.02%。从231份ELISA检测阳性血清中随机抽取了20份,采用RT-PCR方法进行检测,结果共检出18份样品为BVDV/MV RT-PCR阳性,阴性血清未扩增出E0基因,RT-PCR方法检测结果与ELISA方法符合率为90%。
327 serumsamplees were collected from yaks in Tianjun county in Qinghai Provence anddetected with block - ELISA and RT - PCR method. The result showed that 231 of them are positive, positive rate of serum is 70.64%. 110 positive serum has been detected in 135 serum samples of wild yaks, the positive rate is 81.48% ; 121 positive serum has been detected in 192 serum samples by ELISA Randomly and are detected by RT- PCR mehod, 18 is BVDV/MV RT - PCR positive, Eo gene are not amplified in negative serum. The results detected by RT - PCR and ELISA methvod has consistency of 90%.
出处
《青海畜牧兽医杂志》
2012年第4期1-3,共3页
Chinese Qinghai Journal of Animal and Veterinary Sciences
基金
青海省农牧厅--省校联合项目(QNH-2010-395)
