摘要
应用体外培养细胞方法观察了甘草锌对成纤维细胞分裂增生的作用。结果表明不同浓度的甘草锌使成纤维细胞贴壁生长率、细胞核分裂系数均高于对照组,形态学发生显著变化。提示甘草锌抗溃疡作用同促进成纤维细胞合成纤维和基质有关。
This paper reports the experimental study of the effect of licorzine on the extracorporeal fibroblast culture.When each milliliter of the nutrient fluid contains 50μg,75μg,100μg of licorzine respectively,the rate of cellular growth and the coefficient of karyokinesis were much higher than those of the control group.There was a significant change in cell morphology.In the experimental group,the cells grew vigorously,and they appeared large,flat,branching,with fusiform or spindle shape in profile.The ratio of fibroblasts and fibrocytes was 5:2.In young fibroblasts processes interwove with each other,the cytoplasm became plump,and the cell nucleolus could be seen clearly. They were actively engaged in protein synthesis for the production of intercellular substance,the cytoplasm appeared relatively homogeneous and was basophilic because of high concentration of granular endoplasmic reticulum.Fibroblasts are considered to be responsible for the formation of the fibers and synthesize most of the amorphous components of the matrix.In the normal control group, the ratio of fibroblasts and fibrocytes was 5:3.In relatively static fibrocytes,the cytoplasm was sparse and slightly acidphilic since the endoplasmic reticulum was scanty.As licorzine has the function of increasing fibroblasts'synthesis of fibers and matrix,it can promote the healing of the gastric ulcer.
基金
本课题为国家自然科学基金资助题目
