摘要
在对黄芪免疫调节有效成分 F3的研究过程中发现:(1)F3加入100u/ml 白介素Ⅱ(rIL-2),其所诱导的 LAK 细胞对人类黑色素瘤 A375P 的杀伤率可达80%,与1000u/ml rIL-2单独使用时的效果(76%)相似,减少 rIL-2用量的90%。(2)使用 F3后,所需 LAK细胞数量减少50%。提示运用其他生物反应调节剂(BRMs)来增强 rIL-2所诱导的 LAK 细胞杀伤活性是一个值得探索的方向。
Success with rIL-2 immunotherapy of human cancer appears to depend on the administration of high doses which are frequently associated with excessive toxicity.Future use of rIL-2 will require certain modifications based on the use of lower doses of rIL-2 without significant loss of antitumor efficacy.The authors tested in vitro the possibility of potentiating the activity of rIL-2 in terms of LAK cell generation.The authors hypothesized that co-incubation of LAK cell precursors with a Chinese herbal extract (F3) of Astragalus membranaceus(an immune modulator currently under study in the authors' laboratory),along with a low concentration of rIL-2 would generate levels of LAK cell activity equivalent to those generated by high concentrations of rIL-2 alone. The authors found:(1)a 10-fold potentiation of rIL-2 activity manifested by tumor cell killing activity of 80% resulting from LAK cell generation with F3 plus 100 u/ml of rIL-2 versus 76% generated by 1000 u/ml of rIL-2 alone;(2)a significant reduction in the number of effector LAK cells required for equicytotoxic reaction following LAK cell generation with F3 plus rIL-2 compared to rIL-2 alone. The authors conclude that potentiation of antitumor activity mediated by rIL-2 in low concen- trations is possible by the concomitant use of another immune modulator such as Astragalus membranaceus.
