摘要
为了降低工程化组织体外构建过程中的传质限制,采用自行研制的灌注生物反应器系统,以人皮肤成纤维细胞为模型细胞,以PET(Poly(ethylene terephthalate))为三维支架,构建工程化真皮组织,考察灌注速率对成纤维细胞生长和胞外基质合成的影响。将人皮肤成纤维细胞灌注接种于PET支架,分别以0.02、0.2、0.8 cm/min的灌注速率培养18 d。结果表明,灌注培养能促进细胞增殖,提高细胞在3D支架中均匀分布;与静态培养和0.02 cm/min的灌注速率相比,在0.2 cm/min和0.8 cm/min的灌注速率下进行培养,提高了细胞的葡萄糖比消耗速率,刺激细胞合成和分泌更多的胶原基质,但增加了流失到培养液中胶原的比例,灌注培养是体外构建工程化真皮组织的有效方法。
To alleviate the mass transport limit during ex vivo tissue engineering, a perfusion culture bioreactor was developed for fabricating dermal substitutes, and the effects of medium perfusion rate on cell proliferation, distribution and extracellular matrix （ECM） deposition was investigated. PET scaffolds seeded with human dermal fibroblasts by perfusion were cultivated for 18 d at flow rates of 0.02, 0.2, 0.8 cm/min, respectively. It was found that perfusion culture favored cell proliferation, aerobic metabolism as well as a uniform distribution of cells and ECM in 3D engineered constructs. In addition, when compared to that at 0. 02 em/min and static culture, perfusion at 0. 2 cm/min and 0. 8 cm/min significantly stimulated glucose consumption and collagen production. However, a greater leakage of collagen content in culture medium was also observed when perfused at 0.2 em/min and 0.8 cm/min. These data suggested that perfusion culture is a promising method for dermal tissue engineering.
出处
《华东理工大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第4期465-471,共7页
Journal of East China University of Science and Technology
基金
国家自然科学基金(31170951)
国家重点实验室专项经费(2060204)
