摘要
目的 探讨三维支架条件下成骨细胞力学响应模型的建立方法。方法 取孔隙500～800μm、密度0．36～0．45g／cm^3的生物衍生骨支架50块，接种Wistar乳鼠第3代成骨细胞悬液，复合培养。其中24块施加表观应变1000με、3Hz、3min／d的止弦载荷，形成成骨细胞力学响应模型，作为加载组，余作为对照组。复合培养3d后，扫描电镜观察加载组成骨细胞形态；于培养后1、4、8、12、16、20、24和28d，采用MTT法检测成骨细胞增殖。结果 扫描电镜下可见支架表面有梭形成骨细胞附着。成骨细胞增殖检测显示，复合培养后1、4、8、12、16、20、24和28d，加载组细胞数始终高于对照组，且培养后12、16、20、24和28d组间差异均有统计学意义（P〈O．05）。结论 成功建立研究成骨细胞力学响应的模型，为在不同表观应变条件下研究成骨细胞的力学响应提供了方便。
Objective To establish a model for studying on mechanical responses of osteoblasts seeded in 3 dimensional（3D） scaffold. Methods Fifty pieces of bio derived cancellous bones, whose holes were 500 to 800μm and density was 0.36 to 0.45 g/cm^3. were obtained as the scaffolds. They were cultured with the third passage suspension of Wistar rat. Twenty four of the 50 scaffolds were constructed under apparent strain sine waveform with amplitude of 1 000 με, frequency of 3 Hz, and duration of 3 min/d, as experimental group. The other scaffolds were control group. After 3 day coculture, osteoblasts were observed with scanning electron microscope. The proliferation of the osteoblasts was checked by MTT on scheduled date. Results Scanning electron microscopic observation showed that osteoblasts attached and spread on the trabeculae, which presented the validity of the model under proper mechanical condition. Experiment showed that mechanical environment promoted the proliferation of osteoblasts. The observation of proliferation of osteoblasts showed that the quantity of osteoblasts in the experimental group was higher than that in the control group 1,4,8,12,16,20,24, and 28 days after culturing. There was significant difference between the two groups 12,16,20,24,and 28 days after culturing（P〈0.05）. Conclusion The establishment of the model can facilitate the study of mechanical responses of osteoblasts under different conditions.
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2006年第1期58-60,共3页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家自然科学基金资助项目(30470438
30570451)
天津市科技发展基金资助项目(05YFJMJCO1900)
关键词
成骨细胞
力学响应
模型
三维支架
Osteoblast Mechanical responses Model Three dimensional scaffold
