摘要
为进一步了解贵州花生地方品种的遗传多样性,合理、高效利用花生资源,用50对SSR引物评价了贵州不同地理来源的68份花生地方品种,结果多态性较好的41对引物共扩增出79个等位基因,平均每个引物1.93个;多态性信息量(PIC)变幅为0.045(PM43)～0.951(PM169);Shannon信息指数变幅为0.2518(PM79)～0.6926(PM188),平均0.5268;Nei遗传多样性指数变幅为0.1699(PM79)～0.4995(PM188),平均0.3556。采用类平均法对欧氏距离聚类分析表明,在遗传距离为0.94时将68个花生地方品种分成2个大类,同一地理来源、同一粒型的地方品种的亲缘关系并不是最近的,表明地方品种间亲缘关系与地理来源关系不大。说明贵州花生地方品种具有丰富遗传多样性。
In order to assess the genetic diversity of peanut in Guizhou province and provide a theoretical basis for breeding,68 accessions of peanut landraces collected from Guizhou province were evaluated by 50 SSR markers.Seventy-nine alleles were amplified by forty-one polymorphic SSR primer pairs.The average number of alleles per locus was 1.93 with a range from 0.045 to 0.951.Total Shannon information index per locus varied widely from 0.2518（PM79） to 0.6826（PM188） with an average value of 0.5268;total Nei’s genetic diversity index per locus varied widely between 0.1699（PM79） and 0.4995（PM188） with an average value of 0.3556.Cluster analysis with UPGMA showed that the landraces could be divided into two groups and the genetic relationship among materials with similar seed sizes was not in accord with their geographical origins.The peanut landrace in Guizhou showed higher genetic diversity.
出处
《作物学报》
CAS
CSCD
北大核心
2012年第8期1387-1396,共10页
Acta Agronomica Sinica
基金
国家科技部农业科技成果转化项目(2009GB2F200334)
国家现代农业产业体系建设项目(CARS-14-贵州综合试验站)
贵州省农业科技支撑项目(黔科合NY字[2011]3015)
贵州省农业攻关项目(黔科合NY字[2009]3004)
贵州省创新能力建设项目(黔科合院所创新[2010]4002)
贵州省农科院专项资金项目(黔农科院院专项[2009]044号)资助
