摘要
在饲料中添加羰基铁喂养大鼠 ,形成肝脏内铁含量不同的铁过载动物模型 ,体外研究肝组织内不同的铁含量对乙醇诱导肝组织氧化损伤的影响 ,探讨乙醇和铁在诱导肝组织损伤过程中的交互作用。结果显示 :饲料中添加铁剂可明显增加铁在肝脏内的蓄积 ,肝脏内铁含量随喂养时间而增加 ,与对照组比较差异有非常显著意义 (P <0 0 1)。肝脏内不同的铁含量均可明显增加乙醇诱导的肝组织脂质过氧化反应。分别与单纯乙醇和相应的单纯铁过载组比较 ,铁过载组肝组织匀浆中加入一定剂量的乙醇 ,肝组织中MDA含量增加 ,GSH含量减少 ,差异有显著性。肝组织内铁含量与MDA成正相关 ,与GSH含量成负相关 ,且均有显著性差异 (P <0 0 5 )。存在剂量 效应关系。提示 :乙醇诱导的肝组织脂质过氧化反应与肝脏内铁的状况关系密切 ,铁可明显增加乙醇诱导的肝组织脂质过氧化反应。铁和乙醇在诱导肝组织脂质过氧化损伤过程中显示有效交互作用。
To observe the effect of difference content of iron in liver on liver damage induced by ethanol and to investigate the interaction between iron and ethanol in inducing liver damage.Feeding rats a chow diet supplements with carbonyl iron developed experimetal iron loaded rats.Different iron loaded liver homogenates were cultrured with certain dose of ethanol.The iron content,oxidative and lipid peroxidation induced by ethanol was analyzed.The result show that compared to control rats,the content of iron in liver from rats feeding a chow diet supplement with 2\^5%(w/w)carbonyl iron for 2 to 6 weeks increased significantly with time depend pattern.Lipid peroxidation of normal liver homogenates incubated with difference dose of etnanolwas not significant difference from control.On the other hand,the MDA content in different iron loaded homogenates cultured with certain dose of ethanol (0\^5ml)distinctly increased and the GSH content drastically decreased compared to the control.There is a dose responseds relation between them.The result suggests that the content of iron in liver play a key role in lipid peroxidation induced by ethanol.There is a synergistic hepatotoxicitic effect between ethanol and iron overload through inducing liver tissue lipid peroxidation.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2000年第7期596-598,共3页
Chinese Journal of Public Health
基金
国家教委研究生科研资助项目
