摘要
为探讨HSP70高表达与细胞热耐力的关系 ,用硫酸镉诱导细胞HSP70高表达 ;RT -PCR检测HSP70mRNA水平 ;观察HSP70水平对细胞热耐力的影响。结果 :硫酸镉作用于细胞 1h后 ,细胞的HSP70水平显著增高 ,HSP70水平高的细胞其热耐力明显强于HSP70水平低的细胞组 ;HSP70水平于细胞热耐力间正相关。结论 :化学因素诱导的HSP70表达增高可以保护热暴露细胞 ,提高细胞热耐力。
To study the relationship between the HSP70 overexpression and the thermotolerance of cells.The HSP70 of K 562 cells were induced by CdSO 4 and the level of HSP70 were detected by RT PCR,we observed the effects of HSP70 to the heat resistanceby MTT.One hour after the CdSO 4 affected the cells,the HSP70 of cells increased much more,the heat resistance ability of the HSP70 high cells were much more strong than that of the HSP70 low cells and there was a positive correlativity between the HSP70 and the heat resistance.The overexpression of HSP70 that induced by chemical factor could enhance the ability of thermotolerance of cells.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2000年第7期587-588,共2页
Chinese Journal of Public Health
