摘要
目的研究温阳法代表方四逆汤及其组成药物不同配伍,对Lewis肺癌小鼠血清血管内皮生长因子(VEGF)表达的影响,及其抗肿瘤复发转移作用。方法采用正交设计确定四逆汤中附子、干姜、炙甘草的不同配伍,共分为17组,C57BL/6小鼠102只,按正交表分为17组,每组6只,Lewis肺癌小鼠模型制备的次日起,各组小鼠每天进行灌胃给药60 mg/kg。第15天脱颈椎处死各组小鼠。瘤组织称重,计算抑瘤率。观察瘤组织病理变化,计数肺表面转移瘤结节数。用双抗体夹心ELISA法检测血清VEGF变化。结果四逆汤对小鼠Lewis肺癌的瘤重有不同程度的影响。第4、6、7、10组小鼠瘤重、瘤重指数明显低于对照组(第1组,P<0.05)和完整方组(第17组,P<0.05),尤以第10组最明显(P<0.01)。就抑瘤率来说,第4、6、7、10组的抑瘤率也是各药物组中最高的。第5、7、11、16组在转移瘤结节数量上与第1组比较有统计学意义(P<0.05),其中第7、11、16组的转移瘤结节数量少于第1组;在血清VEGF的表达上,第2、3、6、7、10、11、12、13、14、16、17组与第1组比较有显著差异(P<0.01)。结论四逆汤中附子、干姜、炙甘草均具有抑制Lewis肺癌小鼠细胞增殖的作用,其配伍以3∶1∶6的比例对Lewis肺癌小鼠细胞增殖抑制最明显。
Objective： Objective To study the influences and anti-relapse and anti-metastasis effects of Sini Tang and its different combinations of medicinals on expression of serum vascular endothelial growth factor （VEGF） in mice with Lewis lung cancer. Methods The effects of different combinations of Fuzi （ Radix Aconiti Lateralis praeparata ）, Ganjiang （Rhizoma Zingiberis ） and prepared Gancao （prepared Radix Gtycyrrhizae） in Sini Tang were observed by using orthogonal design method. C57BL/6 mice （ n = 102 ） were divided into 17 groups according to orthogonal array （each n = 6 ）. On the second day of establishment of Lewis lung cancer model, all groups were intragastrically given medicinals （60 mg/kg） every day. On the 15th day, all mice were sacrificed by cervical dislocation. The tumor inhibitory rate was calculated after weighing tumor tissue. The pathological changes of tumor tissue were observed, number of metastatic nodules of pulmonary serface was counted, and changes of serum VEGF were observed by using double-antibody sandwich ELISA. Results Sini Tang had influence in varying degrees on tumor weigh. In group 4, 6, 7 and 10, the tumor weight and tumor weight index were significantly lower than those in group 1 （ control group, P 〈 0.05 ） and in group 17 （ complete Sini Tang group, P 〈0.05）, and were most significant in group 10 （P 〈0.01 ）. The tumor inhibitory rate was the highest in group 4, 6, 7 and 10. The number of metastatic nodules had statistical significance in the comparison between group 5,7, 11 and 16 and group 1 （P 〈0.05） , and less in group 7,11 and 16 than that in group 1. The expression of serum VEGF was significantly different in the comparison between group 2,3,6,7,10,11,12,13,14,16 and 17 and group 1 （P〈0.01）. Conclusion In Sini Tang, Fuzi, Ganjiang and prepared Gancao have the inhibitory effect on the proliferation of Lewis lung cancer, and the effect is the most significant when the combination of these three medicinals in the proportion of 3： 1： 6.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2012年第7期470-474,共5页
Journal of Beijing University of Traditional Chinese Medicine
基金
苏州吴中医药中西医结合科研基金资助项目(No.SZD09152)
关键词
四逆汤
肺癌
血管内皮生长因子
小鼠
Sini Tang
lung cancer
vascular endothelial growth factor
mice
