摘要
目的:建立骨伤洗剂中乌头碱的HPLC含量测定方法。方法:Agilent C18(250mm×4.6mm,5.0μm)色谱柱和Agilent C18(20mm×4.6mm,5.0μm)预柱;流动相为0.2%冰醋酸(用三乙胺调至pH6.25)-乙腈(70∶30),检测波长:235nm;流速1.0mL·min-1。结果:乌头碱在0.094～2.350μg(r=0.9985)线性关系良好,加样回收率为98.25%,RSD=2.09%。结论:方法简便,结果准确,可用于骨伤洗剂的质量控制。
Objective： To establish a methed for the determination the contents of aconitine in Gushang- Xiji. Methods： An Agilent Cls （250 mm ×4. 6 mm, 5.0μm） Column was used with mobile phase being 0. 2% Acetic adjuested with Triethylamine to pH 6. 25-Acetonitrile （70：30）, detecting wavelength of 235 nm. Results： Linearities was good at 0. 094 - 2. 350 μg（ r = 0. 9 985 ）. The average recover rate was 98.25% （ RSD = 2. 09% ） Conclusion： The method is accurate and reliable, can be used to determinate aconitine in Gushang-Xiji.
出处
《中国现代中药》
CAS
2012年第8期55-56,共2页
Modern Chinese Medicine
